TY - JOUR
T1 - Red, green, and blue gray-value shift-based approach to whole-field imaging for tissue diagnostics
AU - Krishnan, Mohankumar Valiyambath
AU - Matham, Murukeshan Vadakke
AU - Krishnan, Sathiyamoorthy
AU - Parasuraman, Padmanabhan
AU - Joseph, James
AU - Bhakoo, Kishore
PY - 2012/7/6
Y1 - 2012/7/6
N2 - Identification of abnormal pathology in situ remains one of the challenges of medicine. The interpretation of tissue conditions relies mainly on optical assessment, which can be difficult due to inadequate visual differences or poor color delineation. We propose a methodology to identify regions of abnormal tissue in a targeted area based on red, green, blue (RGB) shift analysis employing a simple CCD color camera and light-emitting diode illumination in a whole-field-imaging scheme. The concept involves analysis of RGB components in an image with respect to a reference set of RGB values under different illumination wavelengths. The magnitude of the gray value shift is estimated by calculating the Euclidean distance between their normalized RGB coordinates. The shift values obtained using these concepts are thereafter used to construct pseudo-colored images with high contrast, enabling easy identification of abnormal areas in the tissue. Images processed from experiments conducted with excised Wistar rat colon sample (lightly doped with Alexafluor 488) and with simulated tumor (cancer cell pellet placed on colon) showed clear localization of tumor region. This proposed approach and methodology is expected to find potential applications for the in vivo diagnosis of disease.
AB - Identification of abnormal pathology in situ remains one of the challenges of medicine. The interpretation of tissue conditions relies mainly on optical assessment, which can be difficult due to inadequate visual differences or poor color delineation. We propose a methodology to identify regions of abnormal tissue in a targeted area based on red, green, blue (RGB) shift analysis employing a simple CCD color camera and light-emitting diode illumination in a whole-field-imaging scheme. The concept involves analysis of RGB components in an image with respect to a reference set of RGB values under different illumination wavelengths. The magnitude of the gray value shift is estimated by calculating the Euclidean distance between their normalized RGB coordinates. The shift values obtained using these concepts are thereafter used to construct pseudo-colored images with high contrast, enabling easy identification of abnormal areas in the tissue. Images processed from experiments conducted with excised Wistar rat colon sample (lightly doped with Alexafluor 488) and with simulated tumor (cancer cell pellet placed on colon) showed clear localization of tumor region. This proposed approach and methodology is expected to find potential applications for the in vivo diagnosis of disease.
KW - Abnormality localization
KW - Disease diagnostics
KW - Fluorescence
KW - RGB shift
KW - Whole field imaging
UR - http://www.scopus.com/inward/record.url?scp=84894072347&partnerID=8YFLogxK
U2 - 10.1117/1.JBO.17.7.076010
DO - 10.1117/1.JBO.17.7.076010
M3 - Article
C2 - 22894493
AN - SCOPUS:84894072347
VL - 17
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 7
M1 - 076010
ER -