Reduction of nitrite to nitric oxide catalyzed by xanthine oxidoreductase

Ben L. Godber, Justin J. Doel, Gopal P. Sapkota, David R. Blake, Cliff R. Stevens, Robert Eisenthal, Roger Harrison

    Research output: Contribution to journalArticlepeer-review

    345 Citations (Scopus)


    Xanthine oxidase (XO) was shown to catalyze the reduction of nitrite to nitric oxide (NO), under anaerobic conditions, in the presence of either NADH or xanthine as reducing substrate. NO production was directly demonstrated by ozone chemiluminescence and showed stoichiometry of approximately 2:1 versus NADH depletion. With xanthine as reducing substrate, the kinetics of NO production were complicated by enzyme inactivation, resulting from NO-induced conversion of XO to its relatively inactive desulfo-form. Steady-state kinetic parameters were determined spectrophotometrically for urate production and NADH oxidation catalyzed by XO and xanthine dehydrogenase in the presence of nitrite under anaerobic conditions. pH optima for anaerobic NO production catalyzed by XO in the presence of nitrite were 7.0 for NADH and
    Original languageEnglish
    Pages (from-to)7757-7763
    Number of pages7
    JournalJournal of Biological Chemistry
    Issue number11
    Publication statusPublished - 17 Mar 2000


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