Reduction of nitrite to nitric oxide catalyzed by xanthine oxidoreductase

Ben L. Godber; Justin J. Doel; Gopal P. Sapkota; David R. Blake; Cliff R. Stevens; Robert Eisenthal; Roger Harrison

doi:10.1074/jbc.275.11.7757

Reduction of nitrite to nitric oxide catalyzed by xanthine oxidoreductase

Ben L. Godber, Justin J. Doel, Gopal P. Sapkota, David R. Blake, Cliff R. Stevens, Robert Eisenthal, Roger Harrison

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Abstract

Xanthine oxidase (XO) was shown to catalyze the reduction of nitrite to nitric oxide (NO), under anaerobic conditions, in the presence of either NADH or xanthine as reducing substrate. NO production was directly demonstrated by ozone chemiluminescence and showed stoichiometry of approximately 2:1 versus NADH depletion. With xanthine as reducing substrate, the kinetics of NO production were complicated by enzyme inactivation, resulting from NO-induced conversion of XO to its relatively inactive desulfo-form. Steady-state kinetic parameters were determined spectrophotometrically for urate production and NADH oxidation catalyzed by XO and xanthine dehydrogenase in the presence of nitrite under anaerobic conditions. pH optima for anaerobic NO production catalyzed by XO in the presence of nitrite were 7.0 for NADH and

Original language	English
Pages (from-to)	7757-7763
Number of pages	7
Journal	Journal of Biological Chemistry
Volume	275
Issue number	11
DOIs	https://doi.org/10.1074/jbc.275.11.7757
Publication status	Published - 17 Mar 2000

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title = "Reduction of nitrite to nitric oxide catalyzed by xanthine oxidoreductase",

abstract = "Xanthine oxidase (XO) was shown to catalyze the reduction of nitrite to nitric oxide (NO), under anaerobic conditions, in the presence of either NADH or xanthine as reducing substrate. NO production was directly demonstrated by ozone chemiluminescence and showed stoichiometry of approximately 2:1 versus NADH depletion. With xanthine as reducing substrate, the kinetics of NO production were complicated by enzyme inactivation, resulting from NO-induced conversion of XO to its relatively inactive desulfo-form. Steady-state kinetic parameters were determined spectrophotometrically for urate production and NADH oxidation catalyzed by XO and xanthine dehydrogenase in the presence of nitrite under anaerobic conditions. pH optima for anaerobic NO production catalyzed by XO in the presence of nitrite were 7.0 for NADH and ",

author = "Godber, {Ben L.} and Doel, {Justin J.} and Sapkota, {Gopal P.} and Blake, {David R.} and Stevens, {Cliff R.} and Robert Eisenthal and Roger Harrison",

year = "2000",

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doi = "10.1074/jbc.275.11.7757",

language = "English",

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TY - JOUR

T1 - Reduction of nitrite to nitric oxide catalyzed by xanthine oxidoreductase

AU - Godber, Ben L.

AU - Doel, Justin J.

AU - Sapkota, Gopal P.

AU - Blake, David R.

AU - Stevens, Cliff R.

AU - Eisenthal, Robert

AU - Harrison, Roger

PY - 2000/3/17

Y1 - 2000/3/17

N2 - Xanthine oxidase (XO) was shown to catalyze the reduction of nitrite to nitric oxide (NO), under anaerobic conditions, in the presence of either NADH or xanthine as reducing substrate. NO production was directly demonstrated by ozone chemiluminescence and showed stoichiometry of approximately 2:1 versus NADH depletion. With xanthine as reducing substrate, the kinetics of NO production were complicated by enzyme inactivation, resulting from NO-induced conversion of XO to its relatively inactive desulfo-form. Steady-state kinetic parameters were determined spectrophotometrically for urate production and NADH oxidation catalyzed by XO and xanthine dehydrogenase in the presence of nitrite under anaerobic conditions. pH optima for anaerobic NO production catalyzed by XO in the presence of nitrite were 7.0 for NADH and

AB - Xanthine oxidase (XO) was shown to catalyze the reduction of nitrite to nitric oxide (NO), under anaerobic conditions, in the presence of either NADH or xanthine as reducing substrate. NO production was directly demonstrated by ozone chemiluminescence and showed stoichiometry of approximately 2:1 versus NADH depletion. With xanthine as reducing substrate, the kinetics of NO production were complicated by enzyme inactivation, resulting from NO-induced conversion of XO to its relatively inactive desulfo-form. Steady-state kinetic parameters were determined spectrophotometrically for urate production and NADH oxidation catalyzed by XO and xanthine dehydrogenase in the presence of nitrite under anaerobic conditions. pH optima for anaerobic NO production catalyzed by XO in the presence of nitrite were 7.0 for NADH and

U2 - 10.1074/jbc.275.11.7757

DO - 10.1074/jbc.275.11.7757

M3 - Article

C2 - 10713088

SN - 0021-9258

VL - 275

SP - 7757

EP - 7763

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 11

ER -

Reduction of nitrite to nitric oxide catalyzed by xanthine oxidoreductase

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