Refined structure of the lipophosphoglycan of Leishmania-donovani

Jerry R. Thomas, Malcolm J. McConville, Jane E. Thomas-Oates, Steven W. Homans, Michael A. J. Ferguson, Philip A. J. Gorin, Kenneth D. Greis, Salvatore J. Turco

    Research output: Contribution to journalArticle

    62 Citations (Scopus)

    Abstract

    The primary structure of the major surface glycoconjugate of Leishmania donovani parasites, a lipophosphoglycan, has been further characterized. The repeating PO4-6Galpß1-4Man disaccharide units, which are a salient feature of the molecule, are shown to terminate with one of several neutral structures, the most abundant of which is the branched trisaccharide Galpß1-4(Manpa1-2)Man. The phosphosaccharide core of lipophosphoglycan, which links the disaccharide repeats to a lipid anchor, contains 2 phosphate residues. One of the core phosphates has previously been localized on O-6 of the galactosyl residue distal to the lipid anchor; the second phosphate is now shown to be on O-6 of the mannosyl residue distal to the anchor and to bear an a-linked glucopyranosyl residue. Also, the anomeric configuration of the unusual 3-substituted Galf residue in the phosphosaccharide core is established as ß. The complete structure of the core is thus PO4-6Galpa1-6Galpa1-3Galfß1-3[Glcpa1-PO4-6]Manpa1-3Manpa1-4GlcNa1-. This further clarification of the structure of lipophosphoglycan may prove beneficial in determining the structure-function relationships of this highly unusual glycoconjugate.

    Original languageEnglish
    Pages (from-to)6829-6833
    Number of pages5
    JournalJournal of Biological Chemistry
    Volume267
    Issue number10
    Publication statusPublished - 5 Apr 1992

    Keywords

    • FAMILY
    • MOIETY
    • ASSIGNMENT
    • H-1-NMR SPECTRA
    • HARTMANN-HAHN SPECTROSCOPY
    • MAJOR PROMASTIGOTES
    • GLYCOLIPIDS
    • GALACTOFURANOSE

    Cite this

    Thomas, J. R., McConville, M. J., Thomas-Oates, J. E., Homans, S. W., Ferguson, M. A. J., Gorin, P. A. J., ... Turco, S. J. (1992). Refined structure of the lipophosphoglycan of Leishmania-donovani. Journal of Biological Chemistry, 267(10), 6829-6833.
    Thomas, Jerry R. ; McConville, Malcolm J. ; Thomas-Oates, Jane E. ; Homans, Steven W. ; Ferguson, Michael A. J. ; Gorin, Philip A. J. ; Greis, Kenneth D. ; Turco, Salvatore J. / Refined structure of the lipophosphoglycan of Leishmania-donovani. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 10. pp. 6829-6833.
    @article{dc7eeece8500437b86fc815f65c6cb46,
    title = "Refined structure of the lipophosphoglycan of Leishmania-donovani",
    abstract = "The primary structure of the major surface glycoconjugate of Leishmania donovani parasites, a lipophosphoglycan, has been further characterized. The repeating PO4-6Galp{\ss}1-4Man disaccharide units, which are a salient feature of the molecule, are shown to terminate with one of several neutral structures, the most abundant of which is the branched trisaccharide Galp{\ss}1-4(Manpa1-2)Man. The phosphosaccharide core of lipophosphoglycan, which links the disaccharide repeats to a lipid anchor, contains 2 phosphate residues. One of the core phosphates has previously been localized on O-6 of the galactosyl residue distal to the lipid anchor; the second phosphate is now shown to be on O-6 of the mannosyl residue distal to the anchor and to bear an a-linked glucopyranosyl residue. Also, the anomeric configuration of the unusual 3-substituted Galf residue in the phosphosaccharide core is established as {\ss}. The complete structure of the core is thus PO4-6Galpa1-6Galpa1-3Galf{\ss}1-3[Glcpa1-PO4-6]Manpa1-3Manpa1-4GlcNa1-. This further clarification of the structure of lipophosphoglycan may prove beneficial in determining the structure-function relationships of this highly unusual glycoconjugate.",
    keywords = "FAMILY, MOIETY, ASSIGNMENT, H-1-NMR SPECTRA, HARTMANN-HAHN SPECTROSCOPY, MAJOR PROMASTIGOTES, GLYCOLIPIDS, GALACTOFURANOSE",
    author = "Thomas, {Jerry R.} and McConville, {Malcolm J.} and Thomas-Oates, {Jane E.} and Homans, {Steven W.} and Ferguson, {Michael A. J.} and Gorin, {Philip A. J.} and Greis, {Kenneth D.} and Turco, {Salvatore J.}",
    year = "1992",
    month = "4",
    day = "5",
    language = "English",
    volume = "267",
    pages = "6829--6833",
    journal = "Journal of Biological Chemistry",
    issn = "0021-9258",
    publisher = "American Society for Biochemistry and Molecular Biology",
    number = "10",

    }

    Thomas, JR, McConville, MJ, Thomas-Oates, JE, Homans, SW, Ferguson, MAJ, Gorin, PAJ, Greis, KD & Turco, SJ 1992, 'Refined structure of the lipophosphoglycan of Leishmania-donovani', Journal of Biological Chemistry, vol. 267, no. 10, pp. 6829-6833.

    Refined structure of the lipophosphoglycan of Leishmania-donovani. / Thomas, Jerry R.; McConville, Malcolm J.; Thomas-Oates, Jane E.; Homans, Steven W.; Ferguson, Michael A. J.; Gorin, Philip A. J.; Greis, Kenneth D.; Turco, Salvatore J.

    In: Journal of Biological Chemistry, Vol. 267, No. 10, 05.04.1992, p. 6829-6833.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Refined structure of the lipophosphoglycan of Leishmania-donovani

    AU - Thomas, Jerry R.

    AU - McConville, Malcolm J.

    AU - Thomas-Oates, Jane E.

    AU - Homans, Steven W.

    AU - Ferguson, Michael A. J.

    AU - Gorin, Philip A. J.

    AU - Greis, Kenneth D.

    AU - Turco, Salvatore J.

    PY - 1992/4/5

    Y1 - 1992/4/5

    N2 - The primary structure of the major surface glycoconjugate of Leishmania donovani parasites, a lipophosphoglycan, has been further characterized. The repeating PO4-6Galpß1-4Man disaccharide units, which are a salient feature of the molecule, are shown to terminate with one of several neutral structures, the most abundant of which is the branched trisaccharide Galpß1-4(Manpa1-2)Man. The phosphosaccharide core of lipophosphoglycan, which links the disaccharide repeats to a lipid anchor, contains 2 phosphate residues. One of the core phosphates has previously been localized on O-6 of the galactosyl residue distal to the lipid anchor; the second phosphate is now shown to be on O-6 of the mannosyl residue distal to the anchor and to bear an a-linked glucopyranosyl residue. Also, the anomeric configuration of the unusual 3-substituted Galf residue in the phosphosaccharide core is established as ß. The complete structure of the core is thus PO4-6Galpa1-6Galpa1-3Galfß1-3[Glcpa1-PO4-6]Manpa1-3Manpa1-4GlcNa1-. This further clarification of the structure of lipophosphoglycan may prove beneficial in determining the structure-function relationships of this highly unusual glycoconjugate.

    AB - The primary structure of the major surface glycoconjugate of Leishmania donovani parasites, a lipophosphoglycan, has been further characterized. The repeating PO4-6Galpß1-4Man disaccharide units, which are a salient feature of the molecule, are shown to terminate with one of several neutral structures, the most abundant of which is the branched trisaccharide Galpß1-4(Manpa1-2)Man. The phosphosaccharide core of lipophosphoglycan, which links the disaccharide repeats to a lipid anchor, contains 2 phosphate residues. One of the core phosphates has previously been localized on O-6 of the galactosyl residue distal to the lipid anchor; the second phosphate is now shown to be on O-6 of the mannosyl residue distal to the anchor and to bear an a-linked glucopyranosyl residue. Also, the anomeric configuration of the unusual 3-substituted Galf residue in the phosphosaccharide core is established as ß. The complete structure of the core is thus PO4-6Galpa1-6Galpa1-3Galfß1-3[Glcpa1-PO4-6]Manpa1-3Manpa1-4GlcNa1-. This further clarification of the structure of lipophosphoglycan may prove beneficial in determining the structure-function relationships of this highly unusual glycoconjugate.

    KW - FAMILY

    KW - MOIETY

    KW - ASSIGNMENT

    KW - H-1-NMR SPECTRA

    KW - HARTMANN-HAHN SPECTROSCOPY

    KW - MAJOR PROMASTIGOTES

    KW - GLYCOLIPIDS

    KW - GALACTOFURANOSE

    M3 - Article

    VL - 267

    SP - 6829

    EP - 6833

    JO - Journal of Biological Chemistry

    JF - Journal of Biological Chemistry

    SN - 0021-9258

    IS - 10

    ER -

    Thomas JR, McConville MJ, Thomas-Oates JE, Homans SW, Ferguson MAJ, Gorin PAJ et al. Refined structure of the lipophosphoglycan of Leishmania-donovani. Journal of Biological Chemistry. 1992 Apr 5;267(10):6829-6833.