Refined structure of the lipophosphoglycan of Leishmania-donovani

Jerry R. Thomas, Malcolm J. McConville, Jane E. Thomas-Oates, Steven W. Homans, Michael A. J. Ferguson, Philip A. J. Gorin, Kenneth D. Greis, Salvatore J. Turco

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    The primary structure of the major surface glycoconjugate of Leishmania donovani parasites, a lipophosphoglycan, has been further characterized. The repeating PO4-6Galpß1-4Man disaccharide units, which are a salient feature of the molecule, are shown to terminate with one of several neutral structures, the most abundant of which is the branched trisaccharide Galpß1-4(Manpa1-2)Man. The phosphosaccharide core of lipophosphoglycan, which links the disaccharide repeats to a lipid anchor, contains 2 phosphate residues. One of the core phosphates has previously been localized on O-6 of the galactosyl residue distal to the lipid anchor; the second phosphate is now shown to be on O-6 of the mannosyl residue distal to the anchor and to bear an a-linked glucopyranosyl residue. Also, the anomeric configuration of the unusual 3-substituted Galf residue in the phosphosaccharide core is established as ß. The complete structure of the core is thus PO4-6Galpa1-6Galpa1-3Galfß1-3[Glcpa1-PO4-6]Manpa1-3Manpa1-4GlcNa1-. This further clarification of the structure of lipophosphoglycan may prove beneficial in determining the structure-function relationships of this highly unusual glycoconjugate.

    Original languageEnglish
    Pages (from-to)6829-6833
    Number of pages5
    JournalJournal of Biological Chemistry
    Issue number10
    Publication statusPublished - 5 Apr 1992


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    Thomas, J. R., McConville, M. J., Thomas-Oates, J. E., Homans, S. W., Ferguson, M. A. J., Gorin, P. A. J., Greis, K. D., & Turco, S. J. (1992). Refined structure of the lipophosphoglycan of Leishmania-donovani. Journal of Biological Chemistry, 267(10), 6829-6833.