Refinement of techniques for the propagation of Leishmania donovani in hamsters

Susan Wyllie, Alan H. Fairlamb (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    19 Citations (Scopus)

    Abstract

    Improved animal models are urgently required for drug and vaccine development against visceral leishmaniasis. Here we report refinements to the hamster model of infection that reduce the severity of the disease as well as the number of animals required to maintain infection while improving parasite yields. A comparison between infection via the intracardiac and intraperitoneal routes showed that the less commonly used intraperitoneal route is the simpler and preferred method. The KAtex latex agglutination test for visceral leishmaniasis accurately detected Leishmania donovani antigen in hamster urine as early as 6 weeks post-inoculation. With modification, this assay could be an important tool in the evaluation of experimental drugs and vaccines.

    Original languageEnglish
    Pages (from-to)364-369
    Number of pages6
    JournalActa Tropica
    Volume97
    Issue number3
    DOIs
    Publication statusPublished - Mar 2006

    Keywords

    • Animal model
    • Hamster
    • Intraperitoneal
    • KAtex
    • Leishmania donovani

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