Refinement of techniques for the propagation of Leishmania donovani in hamsters

Susan Wyllie; Alan H. Fairlamb

doi:10.1016/j.actatropica.2006.01.004

Refinement of techniques for the propagation of Leishmania donovani in hamsters

Susan Wyllie, Alan H. Fairlamb (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Improved animal models are urgently required for drug and vaccine development against visceral leishmaniasis. Here we report refinements to the hamster model of infection that reduce the severity of the disease as well as the number of animals required to maintain infection while improving parasite yields. A comparison between infection via the intracardiac and intraperitoneal routes showed that the less commonly used intraperitoneal route is the simpler and preferred method. The KAtex latex agglutination test for visceral leishmaniasis accurately detected Leishmania donovani antigen in hamster urine as early as 6 weeks post-inoculation. With modification, this assay could be an important tool in the evaluation of experimental drugs and vaccines.

Original language	English
Pages (from-to)	364-369
Number of pages	6
Journal	Acta Tropica
Volume	97
Issue number	3
DOIs	https://doi.org/10.1016/j.actatropica.2006.01.004
Publication status	Published - Mar 2006

Keywords

Animal model
Hamster
Intraperitoneal
KAtex
Leishmania donovani

ASJC Scopus subject areas

Parasitology
veterinary (miscalleneous)
Insect Science
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.actatropica.2006.01.004

Cite this

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title = "Refinement of techniques for the propagation of Leishmania donovani in hamsters",

abstract = "Improved animal models are urgently required for drug and vaccine development against visceral leishmaniasis. Here we report refinements to the hamster model of infection that reduce the severity of the disease as well as the number of animals required to maintain infection while improving parasite yields. A comparison between infection via the intracardiac and intraperitoneal routes showed that the less commonly used intraperitoneal route is the simpler and preferred method. The KAtex latex agglutination test for visceral leishmaniasis accurately detected Leishmania donovani antigen in hamster urine as early as 6 weeks post-inoculation. With modification, this assay could be an important tool in the evaluation of experimental drugs and vaccines.",

keywords = "Animal model, Hamster, Intraperitoneal, KAtex, Leishmania donovani",

author = "Susan Wyllie and Fairlamb, {Alan H.}",

note = "Funding Information: We would like to thank the staff of the Wellcome Trust Resource Unit for their assistance. In addition, we would also like to thank Dr. Luke Newman for helpful discussion and the UK Home Office inspector for suggesting publication of this study. This work was funded by a grant from the Burroughs Wellcome Fund and Wellcome Trust Infectious Disease Initiative.",

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AU - Fairlamb, Alan H.

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N2 - Improved animal models are urgently required for drug and vaccine development against visceral leishmaniasis. Here we report refinements to the hamster model of infection that reduce the severity of the disease as well as the number of animals required to maintain infection while improving parasite yields. A comparison between infection via the intracardiac and intraperitoneal routes showed that the less commonly used intraperitoneal route is the simpler and preferred method. The KAtex latex agglutination test for visceral leishmaniasis accurately detected Leishmania donovani antigen in hamster urine as early as 6 weeks post-inoculation. With modification, this assay could be an important tool in the evaluation of experimental drugs and vaccines.

AB - Improved animal models are urgently required for drug and vaccine development against visceral leishmaniasis. Here we report refinements to the hamster model of infection that reduce the severity of the disease as well as the number of animals required to maintain infection while improving parasite yields. A comparison between infection via the intracardiac and intraperitoneal routes showed that the less commonly used intraperitoneal route is the simpler and preferred method. The KAtex latex agglutination test for visceral leishmaniasis accurately detected Leishmania donovani antigen in hamster urine as early as 6 weeks post-inoculation. With modification, this assay could be an important tool in the evaluation of experimental drugs and vaccines.

KW - Animal model

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KW - Intraperitoneal

KW - KAtex

KW - Leishmania donovani

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Refinement of techniques for the propagation of Leishmania donovani in hamsters

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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