Regulation of Dictyostelium prestalk-specific gene expression by a SHAQKY family MYB transcription factor

Masashi Fukuzawa, Natasha V. Zhukovskaya, Yoko Yamada, Tsuyoshi Araki, Jeffrey G. Williams

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    NOTE: THE SYMBOLS/SPECIAL CHARACTERS IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE ABSTRACT ON THE PUBLISHER’S WEBSITE FOR AN ACCURATE DISPLAY. PstA and pstO cells are the two major populations in the prestalk region of the Dictyostelium slug and DIF-1 is a low molecular weight signalling molecule that selectively induces pstO cell-specific gene expression. The two cell types are defined by their differential use of spatially separated regions of the ecmA promoter. Additionally, there are anterior-like cells (ALCs) scattered throughout the rear, prespore region of the slug. They, like the pstO cells, use a cap-site distal ecmA promoter segment termed the ecmO region. When multimerised, a 22-nucleotide subsegment of the ecmO region directs expression in pstA cells, pstO cells and ALCs. It also directs DIF-inducible gene expression. The 22-nucleotide region was used to purify MybE, a protein with a single MYB DNA-binding domain of a type previously found only in a large family of plant transcription factors. Slugs of a mybE-null (mybE–) strain express an ecmAO:lacZ fusion gene (i.e. a reporter construct containing the ecmA and ecmO promoter regions) in pstA cells but there is little or no expression in pstO cells and ALCs. The ecmA gene is not induced by DIF-1 in a mybE-strain. Thus, MybE is necessary for DIF-1 responsiveness and for the correct differentiation of pstO cells and ALCs.
    Original languageEnglish
    Pages (from-to)1715-1724
    Number of pages10
    JournalDevelopment
    Volume133
    Issue number9
    DOIs
    Publication statusPublished - 2006

    Keywords

    • DIF-1
    • Dictyostelium
    • Prestalk cells
    • SHAQKY family
    • MYB transcription factor

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