Regulation of Dictyostelium prestalk-specific gene expression by a SHAQKY family MYB transcription factor

Masashi Fukuzawa, Natasha V. Zhukovskaya, Yoko Yamada, Tsuyoshi Araki, Jeffrey G. Williams

    Research output: Contribution to journalArticle

    37 Citations (Scopus)

    Abstract

    NOTE: THE SYMBOLS/SPECIAL CHARACTERS IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE ABSTRACT ON THE PUBLISHER’S WEBSITE FOR AN ACCURATE DISPLAY. PstA and pstO cells are the two major populations in the prestalk region of the Dictyostelium slug and DIF-1 is a low molecular weight signalling molecule that selectively induces pstO cell-specific gene expression. The two cell types are defined by their differential use of spatially separated regions of the ecmA promoter. Additionally, there are anterior-like cells (ALCs) scattered throughout the rear, prespore region of the slug. They, like the pstO cells, use a cap-site distal ecmA promoter segment termed the ecmO region. When multimerised, a 22-nucleotide subsegment of the ecmO region directs expression in pstA cells, pstO cells and ALCs. It also directs DIF-inducible gene expression. The 22-nucleotide region was used to purify MybE, a protein with a single MYB DNA-binding domain of a type previously found only in a large family of plant transcription factors. Slugs of a mybE-null (mybE–) strain express an ecmAO:lacZ fusion gene (i.e. a reporter construct containing the ecmA and ecmO promoter regions) in pstA cells but there is little or no expression in pstO cells and ALCs. The ecmA gene is not induced by DIF-1 in a mybE-strain. Thus, MybE is necessary for DIF-1 responsiveness and for the correct differentiation of pstO cells and ALCs.
    Original languageEnglish
    Pages (from-to)1715-1724
    Number of pages10
    JournalDevelopment
    Volume133
    Issue number9
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Dictyostelium
    Transcription Factors
    Gene Expression
    Gastropoda
    Genetic Promoter Regions
    Nucleotides
    Lac Operon
    Cell Differentiation
    Molecular Weight

    Keywords

    • DIF-1
    • Dictyostelium
    • Prestalk cells
    • SHAQKY family
    • MYB transcription factor

    Cite this

    Fukuzawa, Masashi ; Zhukovskaya, Natasha V. ; Yamada, Yoko ; Araki, Tsuyoshi ; Williams, Jeffrey G. / Regulation of Dictyostelium prestalk-specific gene expression by a SHAQKY family MYB transcription factor. In: Development. 2006 ; Vol. 133, No. 9. pp. 1715-1724.
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    abstract = "NOTE: THE SYMBOLS/SPECIAL CHARACTERS IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE ABSTRACT ON THE PUBLISHER’S WEBSITE FOR AN ACCURATE DISPLAY. PstA and pstO cells are the two major populations in the prestalk region of the Dictyostelium slug and DIF-1 is a low molecular weight signalling molecule that selectively induces pstO cell-specific gene expression. The two cell types are defined by their differential use of spatially separated regions of the ecmA promoter. Additionally, there are anterior-like cells (ALCs) scattered throughout the rear, prespore region of the slug. They, like the pstO cells, use a cap-site distal ecmA promoter segment termed the ecmO region. When multimerised, a 22-nucleotide subsegment of the ecmO region directs expression in pstA cells, pstO cells and ALCs. It also directs DIF-inducible gene expression. The 22-nucleotide region was used to purify MybE, a protein with a single MYB DNA-binding domain of a type previously found only in a large family of plant transcription factors. Slugs of a mybE-null (mybE–) strain express an ecmAO:lacZ fusion gene (i.e. a reporter construct containing the ecmA and ecmO promoter regions) in pstA cells but there is little or no expression in pstO cells and ALCs. The ecmA gene is not induced by DIF-1 in a mybE-strain. Thus, MybE is necessary for DIF-1 responsiveness and for the correct differentiation of pstO cells and ALCs.",
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    Regulation of Dictyostelium prestalk-specific gene expression by a SHAQKY family MYB transcription factor. / Fukuzawa, Masashi; Zhukovskaya, Natasha V.; Yamada, Yoko; Araki, Tsuyoshi; Williams, Jeffrey G.

    In: Development, Vol. 133, No. 9, 2006, p. 1715-1724.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Regulation of Dictyostelium prestalk-specific gene expression by a SHAQKY family MYB transcription factor

    AU - Fukuzawa, Masashi

    AU - Zhukovskaya, Natasha V.

    AU - Yamada, Yoko

    AU - Araki, Tsuyoshi

    AU - Williams, Jeffrey G.

    N1 - dc.publisher: Company of Biologists

    PY - 2006

    Y1 - 2006

    N2 - NOTE: THE SYMBOLS/SPECIAL CHARACTERS IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE ABSTRACT ON THE PUBLISHER’S WEBSITE FOR AN ACCURATE DISPLAY. PstA and pstO cells are the two major populations in the prestalk region of the Dictyostelium slug and DIF-1 is a low molecular weight signalling molecule that selectively induces pstO cell-specific gene expression. The two cell types are defined by their differential use of spatially separated regions of the ecmA promoter. Additionally, there are anterior-like cells (ALCs) scattered throughout the rear, prespore region of the slug. They, like the pstO cells, use a cap-site distal ecmA promoter segment termed the ecmO region. When multimerised, a 22-nucleotide subsegment of the ecmO region directs expression in pstA cells, pstO cells and ALCs. It also directs DIF-inducible gene expression. The 22-nucleotide region was used to purify MybE, a protein with a single MYB DNA-binding domain of a type previously found only in a large family of plant transcription factors. Slugs of a mybE-null (mybE–) strain express an ecmAO:lacZ fusion gene (i.e. a reporter construct containing the ecmA and ecmO promoter regions) in pstA cells but there is little or no expression in pstO cells and ALCs. The ecmA gene is not induced by DIF-1 in a mybE-strain. Thus, MybE is necessary for DIF-1 responsiveness and for the correct differentiation of pstO cells and ALCs.

    AB - NOTE: THE SYMBOLS/SPECIAL CHARACTERS IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE ABSTRACT ON THE PUBLISHER’S WEBSITE FOR AN ACCURATE DISPLAY. PstA and pstO cells are the two major populations in the prestalk region of the Dictyostelium slug and DIF-1 is a low molecular weight signalling molecule that selectively induces pstO cell-specific gene expression. The two cell types are defined by their differential use of spatially separated regions of the ecmA promoter. Additionally, there are anterior-like cells (ALCs) scattered throughout the rear, prespore region of the slug. They, like the pstO cells, use a cap-site distal ecmA promoter segment termed the ecmO region. When multimerised, a 22-nucleotide subsegment of the ecmO region directs expression in pstA cells, pstO cells and ALCs. It also directs DIF-inducible gene expression. The 22-nucleotide region was used to purify MybE, a protein with a single MYB DNA-binding domain of a type previously found only in a large family of plant transcription factors. Slugs of a mybE-null (mybE–) strain express an ecmAO:lacZ fusion gene (i.e. a reporter construct containing the ecmA and ecmO promoter regions) in pstA cells but there is little or no expression in pstO cells and ALCs. The ecmA gene is not induced by DIF-1 in a mybE-strain. Thus, MybE is necessary for DIF-1 responsiveness and for the correct differentiation of pstO cells and ALCs.

    KW - DIF-1

    KW - Dictyostelium

    KW - Prestalk cells

    KW - SHAQKY family

    KW - MYB transcription factor

    U2 - 10.1242/dev.02327

    DO - 10.1242/dev.02327

    M3 - Article

    VL - 133

    SP - 1715

    EP - 1724

    JO - Development

    JF - Development

    SN - 0950-1991

    IS - 9

    ER -