Regulation of the activity and expression of ERK8 by DNA damage

TY - JOUR

T1 - Regulation of the activity and expression of ERK8 by DNA damage

AU - Klevernic, Iva V.

AU - Martin, Niall M. B.

AU - Cohen, Philip

PY - 2009/2/18

Y1 - 2009/2/18

N2 - We have investigated the agonists that activate transfected extracellular signal-regulated kinase 8 (ERK8) in cells, and have found that the most potent activators are hydrogen peroxide, DNA alkylating and cross-linking agents and the poly (ADP-ribose) polymerase inhibitor KU-0058948. The feature shared by all these agents is that they lead to the accumulation of single strand breaks in DNA, suggesting a role for ERK8 in the response to, or repair of, DNA single strand breaks. The DNA alkylating agent MMS also induced the disappearance of endogenous ERK8 by a proteasome-dependent mechanism. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

AB - We have investigated the agonists that activate transfected extracellular signal-regulated kinase 8 (ERK8) in cells, and have found that the most potent activators are hydrogen peroxide, DNA alkylating and cross-linking agents and the poly (ADP-ribose) polymerase inhibitor KU-0058948. The feature shared by all these agents is that they lead to the accumulation of single strand breaks in DNA, suggesting a role for ERK8 in the response to, or repair of, DNA single strand breaks. The DNA alkylating agent MMS also induced the disappearance of endogenous ERK8 by a proteasome-dependent mechanism. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

KW - ERK8

KW - MAPK

KW - DNA damage

KW - MMS

U2 - 10.1016/j.febslet.2009.01.011

DO - 10.1016/j.febslet.2009.01.011

M3 - Article

SN - 0014-5793

VL - 583

SP - 680

EP - 684

JO - FEBS Letters

JF - FEBS Letters

IS - 4

ER -