Regulation of vectorial supply of vesicles to the hyphal tip determines thigmotropism in Neurospora crassa

Karen S. Stephenson, Neil A. R. Gow, Fordyce A. Davidson, Geoffrey M. Gadd (Lead / Corresponding author)

    Research output: Contribution to journalArticle

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    Abstract

    Thigmotropism is the ability of an organism to respond to a topographical stimulus by altering its axis of growth. The thigmotropic response of the model fungus Neurospora crassa was quantified using microfabricated glass slides with ridges of defined height. We show that the polarity machinery at the hyphal tip plays a role in the thigmotropic response of N. crassa. Deletion of N. crassa genes encoding the formin, BNI-1, and the Rho-GTPase, CDC-42, an activator of BNI-1 in yeast, CDC-24, its guanine nucleotide exchange factor (GEF), and BEM-1, a scaffold protein in the same pathway, were all shown to significantly decrease the thigmotropic response. In contrast, deletion of genes encoding the cell end-marker protein, TEA-1, and KIP-1, the kinesin responsible for the localisation of TEA-1, significantly increased the thigmotropic response. These results suggest a mechanism of thigmotropism involving vesicle delivery to the hyphal tip via the actin cytoskeleton and microtubules. Neurospora crassa thigmotropic response differed subtly from that of Candida albicans where the stretch-activated calcium channel, Mid1, has been linked with thigmotropic behaviour. The MID-1 deficient mutant of N. crassa (?mid-1) and the effects of calcium depletion were examined here but no change in the thigmotropic response was observed. However, SPRAY, a putative calcium channel protein, was shown to be required for N. crassa thigmotropism. We propose that the thigmotropic response is a result of changes in the polarity machinery at the hyphal tip which are thought to be downstream effects of calcium signalling pathways triggered by mechanical stress at the tip.
    Original languageEnglish
    Pages (from-to)287-294
    Number of pages8
    JournalFungal Biology
    Volume118
    Issue number3
    DOIs
    Publication statusPublished - Mar 2014

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    thigmotropism
    Neurospora crassa
    vesicle
    calcium
    machinery
    protein
    calcium channels
    Calcium Channels
    Centers for Disease Control and Prevention (U.S.)
    gene
    scaffolding proteins
    yeast
    Guanine Nucleotide Exchange Factors
    Kinesin
    kinesin
    rho GTP-Binding Proteins
    Mechanical Stress
    Proteins
    mechanical stress
    Calcium Signaling

    Cite this

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    title = "Regulation of vectorial supply of vesicles to the hyphal tip determines thigmotropism in Neurospora crassa",
    abstract = "Thigmotropism is the ability of an organism to respond to a topographical stimulus by altering its axis of growth. The thigmotropic response of the model fungus Neurospora crassa was quantified using microfabricated glass slides with ridges of defined height. We show that the polarity machinery at the hyphal tip plays a role in the thigmotropic response of N. crassa. Deletion of N. crassa genes encoding the formin, BNI-1, and the Rho-GTPase, CDC-42, an activator of BNI-1 in yeast, CDC-24, its guanine nucleotide exchange factor (GEF), and BEM-1, a scaffold protein in the same pathway, were all shown to significantly decrease the thigmotropic response. In contrast, deletion of genes encoding the cell end-marker protein, TEA-1, and KIP-1, the kinesin responsible for the localisation of TEA-1, significantly increased the thigmotropic response. These results suggest a mechanism of thigmotropism involving vesicle delivery to the hyphal tip via the actin cytoskeleton and microtubules. Neurospora crassa thigmotropic response differed subtly from that of Candida albicans where the stretch-activated calcium channel, Mid1, has been linked with thigmotropic behaviour. The MID-1 deficient mutant of N. crassa (?mid-1) and the effects of calcium depletion were examined here but no change in the thigmotropic response was observed. However, SPRAY, a putative calcium channel protein, was shown to be required for N. crassa thigmotropism. We propose that the thigmotropic response is a result of changes in the polarity machinery at the hyphal tip which are thought to be downstream effects of calcium signalling pathways triggered by mechanical stress at the tip.",
    author = "Stephenson, {Karen S.} and Gow, {Neil A. R.} and Davidson, {Fordyce A.} and Gadd, {Geoffrey M.}",
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    Regulation of vectorial supply of vesicles to the hyphal tip determines thigmotropism in Neurospora crassa. / Stephenson, Karen S.; Gow, Neil A. R.; Davidson, Fordyce A.; Gadd, Geoffrey M. (Lead / Corresponding author).

    In: Fungal Biology, Vol. 118, No. 3, 03.2014, p. 287-294.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Stephenson, Karen S.

    AU - Gow, Neil A. R.

    AU - Davidson, Fordyce A.

    AU - Gadd, Geoffrey M.

    N1 - Copyright © 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

    PY - 2014/3

    Y1 - 2014/3

    N2 - Thigmotropism is the ability of an organism to respond to a topographical stimulus by altering its axis of growth. The thigmotropic response of the model fungus Neurospora crassa was quantified using microfabricated glass slides with ridges of defined height. We show that the polarity machinery at the hyphal tip plays a role in the thigmotropic response of N. crassa. Deletion of N. crassa genes encoding the formin, BNI-1, and the Rho-GTPase, CDC-42, an activator of BNI-1 in yeast, CDC-24, its guanine nucleotide exchange factor (GEF), and BEM-1, a scaffold protein in the same pathway, were all shown to significantly decrease the thigmotropic response. In contrast, deletion of genes encoding the cell end-marker protein, TEA-1, and KIP-1, the kinesin responsible for the localisation of TEA-1, significantly increased the thigmotropic response. These results suggest a mechanism of thigmotropism involving vesicle delivery to the hyphal tip via the actin cytoskeleton and microtubules. Neurospora crassa thigmotropic response differed subtly from that of Candida albicans where the stretch-activated calcium channel, Mid1, has been linked with thigmotropic behaviour. The MID-1 deficient mutant of N. crassa (?mid-1) and the effects of calcium depletion were examined here but no change in the thigmotropic response was observed. However, SPRAY, a putative calcium channel protein, was shown to be required for N. crassa thigmotropism. We propose that the thigmotropic response is a result of changes in the polarity machinery at the hyphal tip which are thought to be downstream effects of calcium signalling pathways triggered by mechanical stress at the tip.

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    DO - 10.1016/j.funbio.2013.12.007

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