Requirement for an upstream element for optimal transcription of a bacterial tRNA gene

Angus I. Lamond, Andrew A. Travers

    Research output: Contribution to journalArticlepeer-review

    115 Citations (Scopus)


    Bacterial promoters are the sites at the 5′ end of each gene that bind RNA polymerase and direct the initiation of transcription. The functional elements of Escherichia coli promoters1,2 are two highly conserved sequences, each about six nucleotides long, usually centred at sites -10 and -35, +1 being the initiating nucleotide. We have been interested in the structure of promoters of genes that are subject to stringent control, that is whose expression is reduced in conditions of amino acid shortage, such as rRNA and tRNA genes. We have therefore mapped the sequences involved in promoting in vivo transcription of a bacterial tRNATyr (tyrT) gene by fusing the tyrT promoter region to a galactokinase (galK) gene, and using in vivo expression of galactokinase activity to measure promoter strength. We show here that efficient expression from the tyrT promoter requires specific sequences upstream of the canonical promoter elements, and we suggest that these sequences constitute an extended promoter structure.

    Original languageEnglish
    Pages (from-to)248-250
    Number of pages3
    Issue number5931
    Publication statusPublished - 15 Sept 1983

    ASJC Scopus subject areas

    • General


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