Response of protein synthesis to anoxia and recovery in anoxia-tolerant hepatocytes

Stephen. C. Land, Leslie T. Buck, Peter W. Hochachka

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Hepatocytes from the western painted turtle (Chrysemys picta bellii) display a profound metabolic suppression under anoxia. Fractional rates of protein synthesis fell by 92% during 12 h anoxia at 25°C and were indistinguishable from the rate obtained with cycloheximide. Normoxic recovery saw protein synthesis increase to 160% of control values and return to normal after 2 h. The GTP-to-GDP ratio, implicated in the control of translation, fell threefold during anoxia. Purine nucleotide phosphate profiles suggest that this change occurs through increasing concentrations of ADP and GDP, with concentrations of ATP and GTP and total purines remaining constant. The normoxic cost for protein synthesis was calculated at 47.6 ± 6.8 mmol ATP/g protein. Normoxic protein synthesis accounted for 36% of overall ATP turnover rates, close to the extent of O2 consumption inhibitable by cycloheximide (28%). Under anoxia, the proportion of ATP turnover utilized by protein synthesis did not change significantly. ATP turnover rates for urea synthesis reflected a similar pattern, falling 72% under anoxia. These results reflect the cell's ability to suppress protein synthesis under anoxia in a manner that is coordinated with the reduction in total metabolic rate.

Original languageEnglish
Pages (from-to)R41-R48
Number of pages8
JournalAmerican Journal of Physiology: Regulatory Integrative and Comparative Physiology
Volume265
Issue number1
DOIs
Publication statusPublished - 1 Jul 1993

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Hepatocytes
Adenosine Triphosphate
Proteins
Cycloheximide
Guanosine Triphosphate
Purine Nucleotides
Purines
Turtles
Hypoxia
Adenosine Diphosphate
Urea
Phosphates
Costs and Cost Analysis

Keywords

  • adenosine 5'-triphosphate turnover
  • Chrysemys picta bellii
  • guanosine 5'-triphosphate-guanosine 5'-diphosphate ratio
  • metabolic suppression
  • protein turnover
  • purine nucleotides
  • urea production
  • western painted turtle

Cite this

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abstract = "Hepatocytes from the western painted turtle (Chrysemys picta bellii) display a profound metabolic suppression under anoxia. Fractional rates of protein synthesis fell by 92{\%} during 12 h anoxia at 25°C and were indistinguishable from the rate obtained with cycloheximide. Normoxic recovery saw protein synthesis increase to 160{\%} of control values and return to normal after 2 h. The GTP-to-GDP ratio, implicated in the control of translation, fell threefold during anoxia. Purine nucleotide phosphate profiles suggest that this change occurs through increasing concentrations of ADP and GDP, with concentrations of ATP and GTP and total purines remaining constant. The normoxic cost for protein synthesis was calculated at 47.6 ± 6.8 mmol ATP/g protein. Normoxic protein synthesis accounted for 36{\%} of overall ATP turnover rates, close to the extent of O2 consumption inhibitable by cycloheximide (28{\%}). Under anoxia, the proportion of ATP turnover utilized by protein synthesis did not change significantly. ATP turnover rates for urea synthesis reflected a similar pattern, falling 72{\%} under anoxia. These results reflect the cell's ability to suppress protein synthesis under anoxia in a manner that is coordinated with the reduction in total metabolic rate.",
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Response of protein synthesis to anoxia and recovery in anoxia-tolerant hepatocytes. / Land, Stephen. C.; Buck, Leslie T.; Hochachka, Peter W.

In: American Journal of Physiology: Regulatory Integrative and Comparative Physiology, Vol. 265, No. 1 , 01.07.1993, p. R41-R48.

Research output: Contribution to journalArticle

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