Ribosomal proteins are targets for the NEDD8 pathway

Dimitris P. Xirodimas, Anders Sundqvist, Akihiro Nakamura, Linnan Shen, Catherine Botting, Ronald T. Hay

    Research output: Contribution to journalArticle

    116 Citations (Scopus)

    Abstract

    Identification of the molecular targets for post-translational modifications is an important step for explaining the regulated pathways. The ubiquitin-like molecule NEDD8 is implicated in the regulation of cell proliferation, viability and development. By combining proteomics and in vivo NEDDylation assays, we identified a subset of ribosomal proteins as novel targets for the NEDD8 pathway. We further show that the lack of NEDDylation in cells causes ribosomal protein instability. Our studies identify a novel and specific role of the NEDD8 pathway in protecting a subset of ribosomal proteins from destabilization.

    Original languageEnglish
    Pages (from-to)280-286
    Number of pages7
    JournalEMBO Reports
    Volume9
    Issue number3
    DOIs
    Publication statusPublished - Mar 2008

    Keywords

    • NEDD8
    • proteomics
    • proteasome
    • ribosomal proteins
    • UBIQUITIN
    • NUCLEOLUS
    • DYNAMICS
    • P53
    • CONJUGATION
    • BIOGENESIS
    • PROTEASE
    • COMPLEX
    • CULLIN
    • NEDP1

    Cite this

    Xirodimas, D. P., Sundqvist, A., Nakamura, A., Shen, L., Botting, C., & Hay, R. T. (2008). Ribosomal proteins are targets for the NEDD8 pathway. EMBO Reports, 9(3), 280-286. https://doi.org/10.1038/embor.2008.10