Ribosomal proteins are targets for the NEDD8 pathway

Dimitris P. Xirodimas; Anders Sundqvist; Akihiro Nakamura; Linnan Shen; Catherine Botting; Ronald T. Hay

doi:10.1038/embor.2008.10

Ribosomal proteins are targets for the NEDD8 pathway

Dimitris P. Xirodimas
, Anders Sundqvist
, Akihiro Nakamura
, Linnan Shen
, Catherine Botting
, Ronald T. Hay

Research output: Contribution to journal › Article › peer-review

153 Citations (Scopus)

Abstract

Identification of the molecular targets for post-translational modifications is an important step for explaining the regulated pathways. The ubiquitin-like molecule NEDD8 is implicated in the regulation of cell proliferation, viability and development. By combining proteomics and in vivo NEDDylation assays, we identified a subset of ribosomal proteins as novel targets for the NEDD8 pathway. We further show that the lack of NEDDylation in cells causes ribosomal protein instability. Our studies identify a novel and specific role of the NEDD8 pathway in protecting a subset of ribosomal proteins from destabilization.

Original language	English
Pages (from-to)	280-286
Number of pages	7
Journal	EMBO Reports
Volume	9
Issue number	3
DOIs	https://doi.org/10.1038/embor.2008.10
Publication status	Published - Mar 2008

Keywords

NEDD8
proteomics
proteasome
ribosomal proteins
UBIQUITIN
NUCLEOLUS
DYNAMICS
P53
CONJUGATION
BIOGENESIS
PROTEASE
COMPLEX
CULLIN
NEDP1

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10.1038/embor.2008.10

http://embor.embopress.org/content/9/3/280

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title = "Ribosomal proteins are targets for the NEDD8 pathway",

abstract = "Identification of the molecular targets for post-translational modifications is an important step for explaining the regulated pathways. The ubiquitin-like molecule NEDD8 is implicated in the regulation of cell proliferation, viability and development. By combining proteomics and in vivo NEDDylation assays, we identified a subset of ribosomal proteins as novel targets for the NEDD8 pathway. We further show that the lack of NEDDylation in cells causes ribosomal protein instability. Our studies identify a novel and specific role of the NEDD8 pathway in protecting a subset of ribosomal proteins from destabilization.",

keywords = "NEDD8, proteomics, proteasome, ribosomal proteins, UBIQUITIN, NUCLEOLUS, DYNAMICS, P53, CONJUGATION, BIOGENESIS, PROTEASE, COMPLEX, CULLIN, NEDP1",

author = "Xirodimas, \{Dimitris P.\} and Anders Sundqvist and Akihiro Nakamura and Linnan Shen and Catherine Botting and Hay, \{Ronald T.\}",

year = "2008",

month = mar,

doi = "10.1038/embor.2008.10",

language = "English",

volume = "9",

pages = "280--286",

journal = "EMBO Reports",

issn = "1469-221X",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "3",

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TY - JOUR

T1 - Ribosomal proteins are targets for the NEDD8 pathway

AU - Xirodimas, Dimitris P.

AU - Sundqvist, Anders

AU - Nakamura, Akihiro

AU - Shen, Linnan

AU - Botting, Catherine

AU - Hay, Ronald T.

PY - 2008/3

Y1 - 2008/3

N2 - Identification of the molecular targets for post-translational modifications is an important step for explaining the regulated pathways. The ubiquitin-like molecule NEDD8 is implicated in the regulation of cell proliferation, viability and development. By combining proteomics and in vivo NEDDylation assays, we identified a subset of ribosomal proteins as novel targets for the NEDD8 pathway. We further show that the lack of NEDDylation in cells causes ribosomal protein instability. Our studies identify a novel and specific role of the NEDD8 pathway in protecting a subset of ribosomal proteins from destabilization.

AB - Identification of the molecular targets for post-translational modifications is an important step for explaining the regulated pathways. The ubiquitin-like molecule NEDD8 is implicated in the regulation of cell proliferation, viability and development. By combining proteomics and in vivo NEDDylation assays, we identified a subset of ribosomal proteins as novel targets for the NEDD8 pathway. We further show that the lack of NEDDylation in cells causes ribosomal protein instability. Our studies identify a novel and specific role of the NEDD8 pathway in protecting a subset of ribosomal proteins from destabilization.

KW - NEDD8

KW - proteomics

KW - proteasome

KW - ribosomal proteins

KW - UBIQUITIN

KW - NUCLEOLUS

KW - DYNAMICS

KW - P53

KW - CONJUGATION

KW - BIOGENESIS

KW - PROTEASE

KW - COMPLEX

KW - CULLIN

KW - NEDP1

U2 - 10.1038/embor.2008.10

DO - 10.1038/embor.2008.10

M3 - Article

C2 - 18274552

SN - 1469-221X

VL - 9

SP - 280

EP - 286

JO - EMBO Reports

JF - EMBO Reports

IS - 3

ER -

Ribosomal proteins are targets for the NEDD8 pathway

Abstract

Keywords

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