TY - JOUR
T1 - RLP/K enrichment sequencing; a novel method to identify receptor-like protein (RLP) and receptor-like kinase (RLK) genes
AU - Lin, Xiao
AU - Armstrong, Miles
AU - Baker, Katie
AU - Wouters, Doret
AU - Visser, Richard G. F.
AU - Wolters, Pieter J.
AU - Hein, Ingo
AU - Vleeshouwers, Vivianne G. A. A.
PY - 2020/5/22
Y1 - 2020/5/22
N2 - The identification of immune receptors in crop plants is time-consuming but important for disease control. Previously, resistance gene enrichment sequencing (RenSeq) was developed to accelerate mapping of nucleotide-binding domain and leucine-rich repeat containing (NLR) genes. However, resistances mediated by pattern recognition receptors (PRRs) remain less utilized. Here, our pipeline shows accelerated mapping of PRRs. Effectoromics leads to precise identification of plants with target PRRs, and subsequent RLP/K enrichment sequencing (RLP/KSeq) leads to detection of informative single nucleotide polymorphisms that are linked to the trait. Using Phytophthora infestans as a model, we identified Solanum microdontum plants that recognize the apoplastic effectors INF1 or SCR74. RLP/KSeq in a segregating Solanum population confirmed the localization of the INF1 receptor on chromosome 12, and led to the rapid mapping of the response to SCR74 to chromosome 9. By using markers obtained from RLP/KSeq in conjunction with additional markers, we fine-mapped the SCR74 receptor to a 43-kbp G-LecRK locus. Our findings show that RLP/KSeq enables rapid mapping of PRRs and is especially beneficial for crop plants with large and complex genomes. This work will enable the elucidation and characterization of the nonNLR plant immune receptors and ultimately facilitate informed resistance breeding.
AB - The identification of immune receptors in crop plants is time-consuming but important for disease control. Previously, resistance gene enrichment sequencing (RenSeq) was developed to accelerate mapping of nucleotide-binding domain and leucine-rich repeat containing (NLR) genes. However, resistances mediated by pattern recognition receptors (PRRs) remain less utilized. Here, our pipeline shows accelerated mapping of PRRs. Effectoromics leads to precise identification of plants with target PRRs, and subsequent RLP/K enrichment sequencing (RLP/KSeq) leads to detection of informative single nucleotide polymorphisms that are linked to the trait. Using Phytophthora infestans as a model, we identified Solanum microdontum plants that recognize the apoplastic effectors INF1 or SCR74. RLP/KSeq in a segregating Solanum population confirmed the localization of the INF1 receptor on chromosome 12, and led to the rapid mapping of the response to SCR74 to chromosome 9. By using markers obtained from RLP/KSeq in conjunction with additional markers, we fine-mapped the SCR74 receptor to a 43-kbp G-LecRK locus. Our findings show that RLP/KSeq enables rapid mapping of PRRs and is especially beneficial for crop plants with large and complex genomes. This work will enable the elucidation and characterization of the nonNLR plant immune receptors and ultimately facilitate informed resistance breeding.
KW - Phytophthora infestans
KW - RLP/K enrichment sequencing (RLP/KSeq)
KW - RenSeq
KW - genotyping by sequencing (GBS)
KW - pattern recognition receptor (PRR)
KW - potato
KW - receptor-like kinase (RLK)
KW - receptor-like protein (RLP)
UR - https://doi.org/10.1101/2020.02.26.966085
UR - http://www.scopus.com/inward/record.url?scp=85084991117&partnerID=8YFLogxK
U2 - 10.1111/nph.16608
DO - 10.1111/nph.16608
M3 - Article
C2 - 32285454
VL - 227
SP - 1264
EP - 1276
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 4
ER -