RNA Silencing Strategies in Phytophthora: Experimental Guidelines and Insights

  • Stephen C. Whisson
  • , Lydia R.J. Welsh
  • , Ramesh R. Vetukuri

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

RNA silencing is a core cellular process that acts to defend the genome against potentially damaging genetic elements such as viruses and transposons. It has been extensively characterized in many eukaryotes and exploited as a tool for determining gene function through removing the activity of specific genes. It has also been used in Phytophthora species to reveal genes involved in different lifecycle stages. In this chapter, we provide guidelines and outline considerations for carrying out RNA silencing experiments in Phytophthora.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsMonica L. Gerth, Rosie E. Bradshaw
PublisherHumana Press
Pages23-34
Number of pages12
ISBN (Electronic)9781071643303
ISBN (Print)9781071643297
DOIs
Publication statusPublished - 28 Dec 2025

Publication series

NameMethods in Molecular Biology
Volume2892
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Gene expression
  • Heterochromatin
  • Phytophthora
  • RNA interference
  • RNA silencing
  • sRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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