TY - JOUR
T1 - Round cells and sperm fertilizing capacity
T2 - the presence of immature germ cells but not seminal leukocytes are associated with reduced success of in vitro fertilization
AU - Tomlinson, Mathew James
AU - Barratt, Christopher Lowther Robert
AU - Bolton, Anthony Ernest
AU - Lenton, Elizabeth Ann
AU - Roberts, Howard Brittan
AU - Cooke, Ian Douglas
PY - 1992/12
Y1 - 1992/12
N2 - Fifty semen samples produced for IVF by patients diagnosed as having unexplained infertility were screened for leukocytes, leukocyte subsets, and immature germ cells using a mAB-based staining procedure. Nonfertilizing ejaculates were found to contain significantly larger numbers of immature germ cells, although no significant differences in leukocyte content were observed between groups. Neither sperm density or progressive motility were significantly different between fertilizing and nonfertilizing groups. We conclude that seminal leukocytes have little if any influence on the fertilizing capacity of the spermatozoa in patients undergoing IVF for unexplained infertility, but the presence of large numbers of germinal elements is associated with reduced fertilizing capacity and may be indicative of an immature sperm population.
AB - Fifty semen samples produced for IVF by patients diagnosed as having unexplained infertility were screened for leukocytes, leukocyte subsets, and immature germ cells using a mAB-based staining procedure. Nonfertilizing ejaculates were found to contain significantly larger numbers of immature germ cells, although no significant differences in leukocyte content were observed between groups. Neither sperm density or progressive motility were significantly different between fertilizing and nonfertilizing groups. We conclude that seminal leukocytes have little if any influence on the fertilizing capacity of the spermatozoa in patients undergoing IVF for unexplained infertility, but the presence of large numbers of germinal elements is associated with reduced fertilizing capacity and may be indicative of an immature sperm population.
KW - Fertilization in Vitro
KW - Humans
KW - Infertility, Male/pathology
KW - Leukocyte Count
KW - Leukocytes/physiology
KW - Male
KW - Semen/cytology
KW - Sperm Count
KW - Sperm Motility
KW - Spermatozoa/physiology
U2 - 10.1016/S0015-0282(16)55583-6
DO - 10.1016/S0015-0282(16)55583-6
M3 - Article
C2 - 1459283
SN - 0015-0282
VL - 58
SP - 1257
EP - 1259
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 6
ER -