Round cells and sperm fertilizing capacity: the presence of immature germ cells but not seminal leukocytes are associated with reduced success of in vitro fertilization

TY - JOUR

T1 - Round cells and sperm fertilizing capacity

T2 - the presence of immature germ cells but not seminal leukocytes are associated with reduced success of in vitro fertilization

AU - Tomlinson, Mathew James

AU - Barratt, Christopher Lowther Robert

AU - Bolton, Anthony Ernest

AU - Lenton, Elizabeth Ann

AU - Roberts, Howard Brittan

AU - Cooke, Ian Douglas

PY - 1992/12

Y1 - 1992/12

N2 - Fifty semen samples produced for IVF by patients diagnosed as having unexplained infertility were screened for leukocytes, leukocyte subsets, and immature germ cells using a mAB-based staining procedure. Nonfertilizing ejaculates were found to contain significantly larger numbers of immature germ cells, although no significant differences in leukocyte content were observed between groups. Neither sperm density or progressive motility were significantly different between fertilizing and nonfertilizing groups. We conclude that seminal leukocytes have little if any influence on the fertilizing capacity of the spermatozoa in patients undergoing IVF for unexplained infertility, but the presence of large numbers of germinal elements is associated with reduced fertilizing capacity and may be indicative of an immature sperm population.

AB - Fifty semen samples produced for IVF by patients diagnosed as having unexplained infertility were screened for leukocytes, leukocyte subsets, and immature germ cells using a mAB-based staining procedure. Nonfertilizing ejaculates were found to contain significantly larger numbers of immature germ cells, although no significant differences in leukocyte content were observed between groups. Neither sperm density or progressive motility were significantly different between fertilizing and nonfertilizing groups. We conclude that seminal leukocytes have little if any influence on the fertilizing capacity of the spermatozoa in patients undergoing IVF for unexplained infertility, but the presence of large numbers of germinal elements is associated with reduced fertilizing capacity and may be indicative of an immature sperm population.

KW - Fertilization in Vitro

KW - Humans

KW - Infertility, Male/pathology

KW - Leukocyte Count

KW - Leukocytes/physiology

KW - Male

KW - Semen/cytology

KW - Sperm Count

KW - Sperm Motility

KW - Spermatozoa/physiology

U2 - 10.1016/S0015-0282(16)55583-6

DO - 10.1016/S0015-0282(16)55583-6

M3 - Article

C2 - 1459283

SN - 0015-0282

VL - 58

SP - 1257

EP - 1259

JO - Fertility and Sterility

JF - Fertility and Sterility

IS - 6

ER -