Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase

Francisco Bustos; Hugo Sepúlveda; Catalina P. Prieto; Margarita Carrasco; Lorena Díaz; José Palma; José Lattus; Martín Montecino; Verónica Palma

doi:10.1002/stem.2704

Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase

Francisco Bustos, Hugo Sepúlveda, Catalina P. Prieto, Margarita Carrasco, Lorena Díaz, José Palma, José Lattus, Martín Montecino (Lead / Corresponding author), Verónica Palma (Lead / Corresponding author)

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Abstract

Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSCs) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the Runt-related transcription factor 2 (RUNX2) osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential. Hence, JARID1B loss of function in WJ-MSC results in increased RUNX2/p57 expression. Our data highlight JARID1B activity as a novel target to modulate WJ-MSC osteoblastic differentiation with potential applications in bone tissue engineering.

Original language	English
Pages (from-to)	2430-2441
Number of pages	12
Journal	Stem Cells
Volume	35
Issue number	12
Early online date	12 Sept 2017
DOIs	https://doi.org/10.1002/stem.2704
Publication status	Published - Dec 2017

Keywords

Epigenetics
Mesenchymal stem cells (MSCs)
Osteoblasts
Umbilical cord

ASJC Scopus subject areas

Molecular Medicine
Developmental Biology
Cell Biology

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10.1002/stem.2704

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Bustos, F., Sepúlveda, H., Prieto, C. P., Carrasco, M., Díaz, L., Palma, J., Lattus, J., Montecino, M., & Palma, V. (2017). Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase. Stem Cells, 35(12), 2430-2441. https://doi.org/10.1002/stem.2704

@article{523031da925b4020a5319b7942d6773f,

title = "Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase",

abstract = "Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSCs) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the Runt-related transcription factor 2 (RUNX2) osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential. Hence, JARID1B loss of function in WJ-MSC results in increased RUNX2/p57 expression. Our data highlight JARID1B activity as a novel target to modulate WJ-MSC osteoblastic differentiation with potential applications in bone tissue engineering.",

keywords = "Epigenetics, Mesenchymal stem cells (MSCs), Osteoblasts, Umbilical cord",

author = "Francisco Bustos and Hugo Sep{\'u}lveda and Prieto, {Catalina P.} and Margarita Carrasco and Lorena D{\'i}az and Jos{\'e} Palma and Jos{\'e} Lattus and Mart{\'i}n Montecino and Ver{\'o}nica Palma",

note = "We acknowledge the MD fellows and personnel of Hospital Luis Tisn{\'e} Brousse for umbilical cord collection, especially doctors S. Aedo, A. Sep{\'u}lveda, C. Berr{\'i}os, F. G{\'a}lvez and M. Acevedo. This work was supported by FONDAP CRG (Center for Genome Regulation) 15090007 to MM and VP, FONDECYT 1130706 to MM, FONDECYT(P)-3140368 to CP , and FONDEF D09E1047 to VP. HS was a recipient of CONICYT doctoral fellowship.",

year = "2017",

month = dec,

doi = "10.1002/stem.2704",

language = "English",

volume = "35",

pages = "2430--2441",

journal = "Stem Cells",

issn = "1066-5099",

publisher = "Oxford University Press",

number = "12",

}

Bustos, F, Sepúlveda, H, Prieto, CP, Carrasco, M, Díaz, L, Palma, J, Lattus, J, Montecino, M & Palma, V 2017, 'Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase', Stem Cells, vol. 35, no. 12, pp. 2430-2441. https://doi.org/10.1002/stem.2704

Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase. / Bustos, Francisco; Sepúlveda, Hugo; Prieto, Catalina P. et al.
In: Stem Cells, Vol. 35, No. 12, 12.2017, p. 2430-2441.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase

AU - Bustos, Francisco

AU - Sepúlveda, Hugo

AU - Prieto, Catalina P.

AU - Carrasco, Margarita

AU - Díaz, Lorena

AU - Palma, José

AU - Lattus, José

AU - Montecino, Martín

AU - Palma, Verónica

N1 - We acknowledge the MD fellows and personnel of Hospital Luis Tisné Brousse for umbilical cord collection, especially doctors S. Aedo, A. Sepúlveda, C. Berríos, F. Gálvez and M. Acevedo. This work was supported by FONDAP CRG (Center for Genome Regulation) 15090007 to MM and VP, FONDECYT 1130706 to MM, FONDECYT(P)-3140368 to CP , and FONDEF D09E1047 to VP. HS was a recipient of CONICYT doctoral fellowship.

PY - 2017/12

Y1 - 2017/12

N2 - Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSCs) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the Runt-related transcription factor 2 (RUNX2) osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential. Hence, JARID1B loss of function in WJ-MSC results in increased RUNX2/p57 expression. Our data highlight JARID1B activity as a novel target to modulate WJ-MSC osteoblastic differentiation with potential applications in bone tissue engineering.

AB - Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSCs) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the Runt-related transcription factor 2 (RUNX2) osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential. Hence, JARID1B loss of function in WJ-MSC results in increased RUNX2/p57 expression. Our data highlight JARID1B activity as a novel target to modulate WJ-MSC osteoblastic differentiation with potential applications in bone tissue engineering.

KW - Epigenetics

KW - Mesenchymal stem cells (MSCs)

KW - Osteoblasts

KW - Umbilical cord

U2 - 10.1002/stem.2704

DO - 10.1002/stem.2704

M3 - Article

C2 - 28895234

AN - SCOPUS:85030457613

SN - 1066-5099

VL - 35

SP - 2430

EP - 2441

JO - Stem Cells

JF - Stem Cells

IS - 12

ER -

Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase

Abstract

Keywords

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