Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase

Francisco Bustos, Hugo Sepúlveda, Catalina P. Prieto, Margarita Carrasco, Lorena Díaz, José Palma, José Lattus, Martín Montecino (Lead / Corresponding author), Verónica Palma (Lead / Corresponding author)

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12 Citations (Scopus)
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Abstract

Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSCs) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the Runt-related transcription factor 2 (RUNX2) osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential. Hence, JARID1B loss of function in WJ-MSC results in increased RUNX2/p57 expression. Our data highlight JARID1B activity as a novel target to modulate WJ-MSC osteoblastic differentiation with potential applications in bone tissue engineering.

Original languageEnglish
Pages (from-to)2430-2441
Number of pages12
JournalStem Cells
Volume35
Issue number12
Early online date12 Sep 2017
DOIs
Publication statusPublished - Dec 2017

Keywords

  • Epigenetics
  • Mesenchymal stem cells (MSCs)
  • Osteoblasts
  • Umbilical cord

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