Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase

Francisco Bustos, Hugo Sepúlveda, Catalina P. Prieto, Margarita Carrasco, Lorena Díaz, José Palma, José Lattus, Martín Montecino (Lead / Corresponding author), Verónica Palma (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSCs) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the Runt-related transcription factor 2 (RUNX2) osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential. Hence, JARID1B loss of function in WJ-MSC results in increased RUNX2/p57 expression. Our data highlight JARID1B activity as a novel target to modulate WJ-MSC osteoblastic differentiation with potential applications in bone tissue engineering.

Original languageEnglish
Pages (from-to)2430-2441
Number of pages12
JournalStem Cells
Volume35
Issue number12
Early online date12 Sep 2017
DOIs
Publication statusPublished - Dec 2017

Fingerprint

Wharton Jelly
Histone Demethylases
Osteoblasts
Mesenchymal Stromal Cells
Transcription Factors
Cell Differentiation
Adult Stem Cells
Bone Regeneration
Umbilical Cord
Tissue Engineering
Regulator Genes
Cell- and Tissue-Based Therapy
Epigenomics
Bone and Bones

Keywords

  • Epigenetics
  • Mesenchymal stem cells (MSCs)
  • Osteoblasts
  • Umbilical cord

Cite this

Bustos, Francisco ; Sepúlveda, Hugo ; Prieto, Catalina P. ; Carrasco, Margarita ; Díaz, Lorena ; Palma, José ; Lattus, José ; Montecino, Martín ; Palma, Verónica. / Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase. In: Stem Cells. 2017 ; Vol. 35, No. 12. pp. 2430-2441.
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abstract = "Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSCs) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the Runt-related transcription factor 2 (RUNX2) osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential. Hence, JARID1B loss of function in WJ-MSC results in increased RUNX2/p57 expression. Our data highlight JARID1B activity as a novel target to modulate WJ-MSC osteoblastic differentiation with potential applications in bone tissue engineering.",
keywords = "Epigenetics, Mesenchymal stem cells (MSCs), Osteoblasts, Umbilical cord",
author = "Francisco Bustos and Hugo Sep{\'u}lveda and Prieto, {Catalina P.} and Margarita Carrasco and Lorena D{\'i}az and Jos{\'e} Palma and Jos{\'e} Lattus and Mart{\'i}n Montecino and Ver{\'o}nica Palma",
note = "We acknowledge the MD fellows and personnel of Hospital Luis Tisn{\'e} Brousse for umbilical cord collection, especially doctors S. Aedo, A. Sep{\'u}lveda, C. Berr{\'i}os, F. G{\'a}lvez and M. Acevedo. This work was supported by FONDAP CRG (Center for Genome Regulation) 15090007 to MM and VP, FONDECYT 1130706 to MM, FONDECYT(P)-3140368 to CP , and FONDEF D09E1047 to VP. HS was a recipient of CONICYT doctoral fellowship.",
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Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase. / Bustos, Francisco; Sepúlveda, Hugo; Prieto, Catalina P.; Carrasco, Margarita; Díaz, Lorena; Palma, José; Lattus, José; Montecino, Martín (Lead / Corresponding author); Palma, Verónica (Lead / Corresponding author).

In: Stem Cells, Vol. 35, No. 12, 12.2017, p. 2430-2441.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Runt-Related Transcription Factor 2 Induction During Differentiation of Wharton's Jelly Mesenchymal Stem Cells to Osteoblasts Is Regulated by Jumonji AT-Rich Interactive Domain 1B Histone Demethylase

AU - Bustos, Francisco

AU - Sepúlveda, Hugo

AU - Prieto, Catalina P.

AU - Carrasco, Margarita

AU - Díaz, Lorena

AU - Palma, José

AU - Lattus, José

AU - Montecino, Martín

AU - Palma, Verónica

N1 - We acknowledge the MD fellows and personnel of Hospital Luis Tisné Brousse for umbilical cord collection, especially doctors S. Aedo, A. Sepúlveda, C. Berríos, F. Gálvez and M. Acevedo. This work was supported by FONDAP CRG (Center for Genome Regulation) 15090007 to MM and VP, FONDECYT 1130706 to MM, FONDECYT(P)-3140368 to CP , and FONDEF D09E1047 to VP. HS was a recipient of CONICYT doctoral fellowship.

PY - 2017/12

Y1 - 2017/12

N2 - Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSCs) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the Runt-related transcription factor 2 (RUNX2) osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential. Hence, JARID1B loss of function in WJ-MSC results in increased RUNX2/p57 expression. Our data highlight JARID1B activity as a novel target to modulate WJ-MSC osteoblastic differentiation with potential applications in bone tissue engineering.

AB - Novel bone regeneration approaches aim to obtain immature osteoblasts from somatic stem cells. Umbilical cord Wharton's jelly mesenchymal stem cells (WJ-MSCs) are an ideal source for cell therapy. Hence, the study of mechanisms involved in WJ-MSC osteoblastic differentiation is crucial to exploit their developmental capacity. Here, we have assessed epigenetic control of the Runt-related transcription factor 2 (RUNX2) osteogenic master regulator gene in WJ-MSC. We present evidence indicating that modulation of RUNX2 expression through preventing Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase activity is relevant to enhance WJ-MSC osteoblastic potential. Hence, JARID1B loss of function in WJ-MSC results in increased RUNX2/p57 expression. Our data highlight JARID1B activity as a novel target to modulate WJ-MSC osteoblastic differentiation with potential applications in bone tissue engineering.

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KW - Mesenchymal stem cells (MSCs)

KW - Osteoblasts

KW - Umbilical cord

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VL - 35

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JO - Stem Cells

JF - Stem Cells

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