Salicylic acid confers resistance to a biotrophic rust pathogen, Puccinia substriata, in pearl millet (Pennisetum glaucum)

Bridget G Crampton, Ingo Hein, Dave K Berger (Lead / Corresponding author)

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Studies were undertaken to assess the induction of defence response pathways in pearl millet (Pennisetum glaucum) in response to infection with the leaf rust fungus Puccinia substriata. Pretreatment of pearl millet with salicylic acid (SA) conferred resistance to a virulent isolate of the rust fungus, whereas methyl jasmonate (MeJA) did not significantly reduce infection levels. These results suggest that the SA defence pathway is involved in rust resistance. In order to identify pearl millet genes that are specifically regulated in response to SA and not MeJA, and thus could play a role in resistance to P. substriata, gene expression profiling was performed. Substantial overlap in gene expression responses between the treatments was observed, with MeJA and SA treatments exhibiting 17% co-regulated transcripts. However, 34% of transcripts were differentially expressed in response to SA treatment, but not in response to MeJA treatment. SA-responsive transcripts represented genes involved in SA metabolism, defence response, signal transduction, protection from oxidative stress and photosynthesis. The expression profiles of pearl millet plants after treatment with SA or MeJA were more similar to one another than to the response during a compatible infection with P. substriata. However, some SA-responsive genes were repressed during P. substriata infection, indicating possible manipulation of host responses by the pathogen.

Original languageEnglish
Pages (from-to)291-304
Number of pages14
JournalMolecular Plant Pathology
Volume10
Issue number2
DOIs
Publication statusPublished - Mar 2009

Fingerprint

Puccinia substriata
Pennisetum
salicylic acid
Salicylic Acid
millet
rust disease
Pennisetum glaucum
pathogen
methyl jasmonate
pathogens
Infection
infection
gene expression
gene
Fungi
fungus
Genes
fungi
genes
leaf rust

Keywords

  • Acetates/pharmacology
  • Basidiomycota/physiology
  • Cluster Analysis
  • Cyclopentanes/pharmacology
  • DNA, Complementary/genetics
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant/drug effects
  • Genes, Plant
  • Immunity, Innate/drug effects
  • Oligonucleotide Array Sequence Analysis
  • Oxylipins/pharmacology
  • Pennisetum/genetics
  • Plant Diseases/genetics
  • Plant Leaves/drug effects
  • Salicylic Acid/pharmacology

Cite this

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title = "Salicylic acid confers resistance to a biotrophic rust pathogen, Puccinia substriata, in pearl millet (Pennisetum glaucum)",
abstract = "Studies were undertaken to assess the induction of defence response pathways in pearl millet (Pennisetum glaucum) in response to infection with the leaf rust fungus Puccinia substriata. Pretreatment of pearl millet with salicylic acid (SA) conferred resistance to a virulent isolate of the rust fungus, whereas methyl jasmonate (MeJA) did not significantly reduce infection levels. These results suggest that the SA defence pathway is involved in rust resistance. In order to identify pearl millet genes that are specifically regulated in response to SA and not MeJA, and thus could play a role in resistance to P. substriata, gene expression profiling was performed. Substantial overlap in gene expression responses between the treatments was observed, with MeJA and SA treatments exhibiting 17{\%} co-regulated transcripts. However, 34{\%} of transcripts were differentially expressed in response to SA treatment, but not in response to MeJA treatment. SA-responsive transcripts represented genes involved in SA metabolism, defence response, signal transduction, protection from oxidative stress and photosynthesis. The expression profiles of pearl millet plants after treatment with SA or MeJA were more similar to one another than to the response during a compatible infection with P. substriata. However, some SA-responsive genes were repressed during P. substriata infection, indicating possible manipulation of host responses by the pathogen.",
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author = "Crampton, {Bridget G} and Ingo Hein and Berger, {Dave K}",
year = "2009",
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Salicylic acid confers resistance to a biotrophic rust pathogen, Puccinia substriata, in pearl millet (Pennisetum glaucum). / Crampton, Bridget G; Hein, Ingo; Berger, Dave K (Lead / Corresponding author).

In: Molecular Plant Pathology, Vol. 10, No. 2, 03.2009, p. 291-304.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Salicylic acid confers resistance to a biotrophic rust pathogen, Puccinia substriata, in pearl millet (Pennisetum glaucum)

AU - Crampton, Bridget G

AU - Hein, Ingo

AU - Berger, Dave K

PY - 2009/3

Y1 - 2009/3

N2 - Studies were undertaken to assess the induction of defence response pathways in pearl millet (Pennisetum glaucum) in response to infection with the leaf rust fungus Puccinia substriata. Pretreatment of pearl millet with salicylic acid (SA) conferred resistance to a virulent isolate of the rust fungus, whereas methyl jasmonate (MeJA) did not significantly reduce infection levels. These results suggest that the SA defence pathway is involved in rust resistance. In order to identify pearl millet genes that are specifically regulated in response to SA and not MeJA, and thus could play a role in resistance to P. substriata, gene expression profiling was performed. Substantial overlap in gene expression responses between the treatments was observed, with MeJA and SA treatments exhibiting 17% co-regulated transcripts. However, 34% of transcripts were differentially expressed in response to SA treatment, but not in response to MeJA treatment. SA-responsive transcripts represented genes involved in SA metabolism, defence response, signal transduction, protection from oxidative stress and photosynthesis. The expression profiles of pearl millet plants after treatment with SA or MeJA were more similar to one another than to the response during a compatible infection with P. substriata. However, some SA-responsive genes were repressed during P. substriata infection, indicating possible manipulation of host responses by the pathogen.

AB - Studies were undertaken to assess the induction of defence response pathways in pearl millet (Pennisetum glaucum) in response to infection with the leaf rust fungus Puccinia substriata. Pretreatment of pearl millet with salicylic acid (SA) conferred resistance to a virulent isolate of the rust fungus, whereas methyl jasmonate (MeJA) did not significantly reduce infection levels. These results suggest that the SA defence pathway is involved in rust resistance. In order to identify pearl millet genes that are specifically regulated in response to SA and not MeJA, and thus could play a role in resistance to P. substriata, gene expression profiling was performed. Substantial overlap in gene expression responses between the treatments was observed, with MeJA and SA treatments exhibiting 17% co-regulated transcripts. However, 34% of transcripts were differentially expressed in response to SA treatment, but not in response to MeJA treatment. SA-responsive transcripts represented genes involved in SA metabolism, defence response, signal transduction, protection from oxidative stress and photosynthesis. The expression profiles of pearl millet plants after treatment with SA or MeJA were more similar to one another than to the response during a compatible infection with P. substriata. However, some SA-responsive genes were repressed during P. substriata infection, indicating possible manipulation of host responses by the pathogen.

KW - Acetates/pharmacology

KW - Basidiomycota/physiology

KW - Cluster Analysis

KW - Cyclopentanes/pharmacology

KW - DNA, Complementary/genetics

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Plant/drug effects

KW - Genes, Plant

KW - Immunity, Innate/drug effects

KW - Oligonucleotide Array Sequence Analysis

KW - Oxylipins/pharmacology

KW - Pennisetum/genetics

KW - Plant Diseases/genetics

KW - Plant Leaves/drug effects

KW - Salicylic Acid/pharmacology

U2 - 10.1111/j.1364-3703.2008.00532.x

DO - 10.1111/j.1364-3703.2008.00532.x

M3 - Article

VL - 10

SP - 291

EP - 304

JO - Molecular Plant Pathology

JF - Molecular Plant Pathology

SN - 1464-6722

IS - 2

ER -