TY - JOUR
T1 - Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection
AU - Sivadasan, Priya
AU - Gupta, Manoj Kumar
AU - Sathe, Gajanan
AU - Sudheendra, H. V.
AU - Sunny, Sumsum P.
AU - Renu, Durairaj
AU - Hari, P. S.
AU - Gowda, H.
AU - Suresh, Amritha
AU - Kuriakose, Moni Abraham
AU - Sirdeshmukh, Ravi
N1 - Funding Information:
This work was supported by the Department of Biotechnology (DBT), Government of India ( BT/PR13505/Med/ 30/276/2010 and BT/01/CEIB/11/IV/05 Dated22/08/13). PS was awarded a Ph.D. from Rajiv Gandhi University of Health Sciences, Bangalore for the work presented in the manuscript. MKG is currently working at Syngene International Limited , Bangalore. Authors thank the organisation for his time.
Funding Information:
This work was supported by the Department of Biotechnology (DBT), Government of India (BT/PR13505/Med/ 30/276/2010 and BT/01/CEIB/11/IV/05 Dated22/08/13). PS was awarded a Ph.D. from Rajiv Gandhi University of Health Sciences, Bangalore for the work presented in the manuscript. MKG is currently working at Syngene International Limited, Bangalore. Authors thank the organisation for his time. Priya Sivadasan: Conceptualization, Methodology, Validation, Formal Analysis, Investigation, Data curation, Writing ? original draft, Visualization, Funding acquisition. Manoj Kumar Gupta: Methodology, Formal Analysis. Gajanan Sathe: Methodology, Formal Analysis. H?V Sudheendra: Validation, Formal Analysis. Sumsum P Sunny: Resources, Data curation. Durairaj Renu: Software, Data Curation, Formal Analysis. Hari P S: Visualization. Harsha Gowda: Methodology, Formal Analysis. Amritha Suresh: Conceptualization, Methodology, Writing- Review & Editing, Supervision, Project Administration, Funding acquisition. Moni Abraham Kuriakose: Conceptualization, Methodology, Writing- Review & Editing, Supervision, Project Administration, Funding acquisition. Ravi Sirdeshmukh: Conceptualization, Methodology, Writing- Review & Editing, Supervision, Project Administration, Funding acquisition.
Publisher Copyright:
© 2019
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/2/10
Y1 - 2020/2/10
N2 - Dysplastic leukoplakia (LP) of the oral cavity is a potentially malignant condition for oral squamous cell carcinoma (OSCC), early detection of which remains an unmet clinical need. In an effort to develop non-invasive biomarker based method for early detection of the disease, differential proteomic profiling was carried out with the saliva from patients with risk habits and diagnosed with LP and those with lymph node negative and positive OSCC in comparison to healthy controls with risk habits. Ninety three proteins were observed at elevated level (≥1.5 fold), and 30 were prioritized based on a scoring system comprising of confidence of identification, presence in the various specimen groups, functional relevance, and their secretory potential. Verification was carried out in independent patient cohorts for 8 selected, representative, upregulated proteins using ELISA. Three of them CD44, S100A7, and S100P were significantly altered in patients with LP as well as OSCC and can be regarded as a panel of biomarker candidates for early detection of the malignancy. Other members may also be investigated in a targeted manner to expand the portfolio of biomarkers for early detection. The mass spectrometry data are available via ProteomeXchange with identifier PXD015722. Significance: There is an unmet clinical need for non-invasive, biomarker based methods for the improved early detection and the subsequent management of oral cancer. The study represents differential proteome profiling of the saliva of patients with oral dysplastic leukoplakia (LP) – a potentially malignant lesion, patients diagnosed with oral squamous cell carcinoma (OSCC), and healthy controls to identify potential markers for the purpose of early detection of malignancy. From among the matched and prioritized proteins with elevated levels in the saliva of patients with LP and those with OSCC, eight were verified. Three of them – CD44, S100A7 and S100P appeared promising candidates as biomarkers for early detection of the neoplastic predisposition and may form the basis of clinical assays for this purpose.
AB - Dysplastic leukoplakia (LP) of the oral cavity is a potentially malignant condition for oral squamous cell carcinoma (OSCC), early detection of which remains an unmet clinical need. In an effort to develop non-invasive biomarker based method for early detection of the disease, differential proteomic profiling was carried out with the saliva from patients with risk habits and diagnosed with LP and those with lymph node negative and positive OSCC in comparison to healthy controls with risk habits. Ninety three proteins were observed at elevated level (≥1.5 fold), and 30 were prioritized based on a scoring system comprising of confidence of identification, presence in the various specimen groups, functional relevance, and their secretory potential. Verification was carried out in independent patient cohorts for 8 selected, representative, upregulated proteins using ELISA. Three of them CD44, S100A7, and S100P were significantly altered in patients with LP as well as OSCC and can be regarded as a panel of biomarker candidates for early detection of the malignancy. Other members may also be investigated in a targeted manner to expand the portfolio of biomarkers for early detection. The mass spectrometry data are available via ProteomeXchange with identifier PXD015722. Significance: There is an unmet clinical need for non-invasive, biomarker based methods for the improved early detection and the subsequent management of oral cancer. The study represents differential proteome profiling of the saliva of patients with oral dysplastic leukoplakia (LP) – a potentially malignant lesion, patients diagnosed with oral squamous cell carcinoma (OSCC), and healthy controls to identify potential markers for the purpose of early detection of malignancy. From among the matched and prioritized proteins with elevated levels in the saliva of patients with LP and those with OSCC, eight were verified. Three of them – CD44, S100A7 and S100P appeared promising candidates as biomarkers for early detection of the neoplastic predisposition and may form the basis of clinical assays for this purpose.
KW - Early detection
KW - Leukoplakia
KW - Oral Cancer
KW - Oral squamous cell carcinoma
KW - Proteomics
KW - Salivary biomarkers
UR - http://www.scopus.com/inward/record.url?scp=85074896367&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2019.103574
DO - 10.1016/j.jprot.2019.103574
M3 - Article
C2 - 31706945
AN - SCOPUS:85074896367
SN - 1874-3919
VL - 212
JO - Journal of Proteomics
JF - Journal of Proteomics
M1 - 103574
ER -
