Projects per year
Significance Statement Proper dendritic growth is a critical step in the development of neuronal connectivity that underlies proper neuronal communication. Much is known about how NMDA receptors drive neuronal development and plasticity, but less is known about how AMPA receptors contribute in an independent manner. While SAP97 plays a critical role in this process, the molecular mechanisms and binding partners that subserve these effects are under active exploration. Here we show that the cysteine-rich interactor of PDZ3 (CRIPT) is a bona fide binding partner of SAP97 in biochemical assays and resides in dendrites in the vicinity of putative AMPAergic synapses. In knockdown experiments, we find that CRIPT is essential for SAP97-dependent dendrite growth in vitro. We extend these studies to an in vivo model and show that CRIPT is also essential for dendrite growth and mechanosensory function in C.elegans. This work links AMPA receptors, MAGUKs and CRIPT to essential neuronal cell biology and C.elegans behavior.
Sonopill: Minimally Invasive Gastrointestinal Diagnosis and Therapy (Joint with University of Glasgow & Heriot Watt University)
27/05/13 → 14/12/18