Scaffold-free, size-controlled generation of spheroids for biochemical assays, drug screening and high-content imaging: Protocol

Ralitsa Madsen

doi:10.17504/protocols.io.3byl4bnrrvo5/v1

Scaffold-free, size-controlled generation of spheroids for biochemical assays, drug screening and high-content imaging: Protocol

Ralitsa Madsen

MRC PPU

Research output: Other contribution

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Abstract

This protocol will allow the user to establish size-controlled, scaffold-free spheroid cultures suitable for drug screening, biochemical signalling assays and high-content imaging. This protocol has been tested on and worked successfully with the following cell lines: HeLa, MCF10A, T47D, BT474.

Original language	English
Type	Research Protocol
Publisher	protocols.io
DOIs	https://doi.org/10.17504/protocols.io.3byl4bnrrvo5/v1
Publication status	Published - 5 Dec 2022

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10.17504/protocols.io.3byl4bnrrvo5/v1Licence: CC BY

Final Published VersionFinal published version, 230 KBLicence: CC BY

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title = "Scaffold-free, size-controlled generation of spheroids for biochemical assays, drug screening and high-content imaging: Protocol",

abstract = "This protocol will allow the user to establish size-controlled, scaffold-free spheroid cultures suitable for drug screening, biochemical signalling assays and high-content imaging. This protocol has been tested on and worked successfully with the following cell lines: HeLa, MCF10A, T47D, BT474.",

author = "Ralitsa Madsen",

note = "Wellcome Trust Grant ID: 220464/Z/20/Z",

year = "2022",

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doi = "10.17504/protocols.io.3byl4bnrrvo5/v1",

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AU - Madsen, Ralitsa

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PY - 2022/12/5

Y1 - 2022/12/5

N2 - This protocol will allow the user to establish size-controlled, scaffold-free spheroid cultures suitable for drug screening, biochemical signalling assays and high-content imaging. This protocol has been tested on and worked successfully with the following cell lines: HeLa, MCF10A, T47D, BT474.

AB - This protocol will allow the user to establish size-controlled, scaffold-free spheroid cultures suitable for drug screening, biochemical signalling assays and high-content imaging. This protocol has been tested on and worked successfully with the following cell lines: HeLa, MCF10A, T47D, BT474.

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DO - 10.17504/protocols.io.3byl4bnrrvo5/v1

M3 - Other contribution

PB - protocols.io

ER -

Scaffold-free, size-controlled generation of spheroids for biochemical assays, drug screening and high-content imaging: Protocol

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