SCF-Fbxo42 promotes synaptonemal complex assembly by downregulating PP2A-B56

Pedro Barbosa; Liudmila Zhaunova; Simona Debilio; Verdiana Steccanella; Van Kelly; Tony Ly; Hiroyuki Ohkura

doi:10.1083/jcb.202009167

SCF-Fbxo42 promotes synaptonemal complex assembly by downregulating PP2A-B56

Pedro Barbosa, Liudmila Zhaunova, Simona Debilio, Verdiana Steccanella, Van Kelly, Tony Ly, Hiroyuki Ohkura (Lead / Corresponding author)

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Abstract

Meiosis creates genetic diversity by recombination and segregation of chromosomes. The synaptonemal complex assembles during meiotic prophase I and assists faithful exchanges between homologous chromosomes, but how its assembly/disassembly is regulated remains to be understood. Here, we report how two major posttranslational modifications, phosphorylation and ubiquitination, cooperate to promote synaptonemal complex assembly. We found that the ubiquitin ligase complex SCF is important for assembly and maintenance of the synaptonemal complex in Drosophila female meiosis. This function of SCF is mediated by two substrate-recognizing F-box proteins, Slmb/βTrcp and Fbxo42. SCF-Fbxo42 downregulates the phosphatase subunit PP2A-B56, which is important for synaptonemal complex assembly and maintenance.

Original language	English
Article number	e202009167
Number of pages	12
Journal	Journal of Cell Biology
Volume	220
Issue number	2
DOIs	https://doi.org/10.1083/jcb.202009167
Publication status	Published - 31 Dec 2020

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10.1083/jcb.202009167Licence: CC BY

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title = "SCF-Fbxo42 promotes synaptonemal complex assembly by downregulating PP2A-B56",

abstract = "Meiosis creates genetic diversity by recombination and segregation of chromosomes. The synaptonemal complex assembles during meiotic prophase I and assists faithful exchanges between homologous chromosomes, but how its assembly/disassembly is regulated remains to be understood. Here, we report how two major posttranslational modifications, phosphorylation and ubiquitination, cooperate to promote synaptonemal complex assembly. We found that the ubiquitin ligase complex SCF is important for assembly and maintenance of the synaptonemal complex in Drosophila female meiosis. This function of SCF is mediated by two substrate-recognizing F-box proteins, Slmb/βTrcp and Fbxo42. SCF-Fbxo42 downregulates the phosphatase subunit PP2A-B56, which is important for synaptonemal complex assembly and maintenance.",

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T1 - SCF-Fbxo42 promotes synaptonemal complex assembly by downregulating PP2A-B56

AU - Barbosa, Pedro

AU - Zhaunova, Liudmila

AU - Debilio, Simona

AU - Steccanella, Verdiana

AU - Kelly, Van

AU - Ly, Tony

AU - Ohkura, Hiroyuki

PY - 2020/12/31

Y1 - 2020/12/31

N2 - Meiosis creates genetic diversity by recombination and segregation of chromosomes. The synaptonemal complex assembles during meiotic prophase I and assists faithful exchanges between homologous chromosomes, but how its assembly/disassembly is regulated remains to be understood. Here, we report how two major posttranslational modifications, phosphorylation and ubiquitination, cooperate to promote synaptonemal complex assembly. We found that the ubiquitin ligase complex SCF is important for assembly and maintenance of the synaptonemal complex in Drosophila female meiosis. This function of SCF is mediated by two substrate-recognizing F-box proteins, Slmb/βTrcp and Fbxo42. SCF-Fbxo42 downregulates the phosphatase subunit PP2A-B56, which is important for synaptonemal complex assembly and maintenance.

AB - Meiosis creates genetic diversity by recombination and segregation of chromosomes. The synaptonemal complex assembles during meiotic prophase I and assists faithful exchanges between homologous chromosomes, but how its assembly/disassembly is regulated remains to be understood. Here, we report how two major posttranslational modifications, phosphorylation and ubiquitination, cooperate to promote synaptonemal complex assembly. We found that the ubiquitin ligase complex SCF is important for assembly and maintenance of the synaptonemal complex in Drosophila female meiosis. This function of SCF is mediated by two substrate-recognizing F-box proteins, Slmb/βTrcp and Fbxo42. SCF-Fbxo42 downregulates the phosphatase subunit PP2A-B56, which is important for synaptonemal complex assembly and maintenance.

U2 - 10.1083/jcb.202009167

DO - 10.1083/jcb.202009167

M3 - Article

C2 - 33382409

SN - 0021-9525

VL - 220

JO - Journal of Cell Biology

JF - Journal of Cell Biology

IS - 2

M1 - e202009167

ER -

SCF-Fbxo42 promotes synaptonemal complex assembly by downregulating PP2A-B56

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