Scotin, a novel p53-inducible proapoptotic protein located in the ER and the nuclear membrane

J.-C. Bourdon, J. Renzing, P. L. Robertson, K. N. Fernandes, D. P. Lane

    Research output: Contribution to journalArticle

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    Abstract

    p53 is a transcription factor that induces growth arrest or apoptosis in response to cellular stress. To identify new p53-inducible proapoptotic genes, we compared, by differential display, the expression of genes in spleen or thymus of normal and p53 nullizygote mice after gamma-irradiation of whole animals. We report the identification and characterization of human and mouse Scotin homologues, a novel gene directly transactivated by p53. The Scotin protein is localized to the ER and the nuclear membrane. Scotin can induce apoptosis in a caspase-dependent manner. Inhibition of endogenous Scotin expression increases resistance to p53-dependent apoptosis induced by DNA damage, suggesting that Scotin plays a role in p53-dependent apoptosis. The discovery of Scotin brings to light a role of the ER in p53-dependent apoptosis.

    © 2002 Bourdon et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

    Original languageEnglish
    Pages (from-to)235-246
    Number of pages12
    JournalJournal of Cell Biology
    Volume158
    Issue number2
    DOIs
    Publication statusPublished - 22 Jul 2002

    Keywords

    • Transactivation
    • p53-binding site
    • Cell death
    • Cancer
    • 3p21

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