Screening Congo Red and its analogues for their ability to prevent the formation of PrP-res in scrapie-infected cells

Helene Rudyk, Snezana Vasiljevic, Ruth M. Hennion, Christopher R. Birkett, James Hope, Ian H. Gilbert

    Research output: Contribution to journalArticlepeer-review

    95 Citations (Scopus)

    Abstract

    Transmissible spongiform encephalopathies (TSEs) are incurable, fatal diseases. The dye Congo Red (CR) can cure cells infected with agents of the sheep TSE, scrapie, but is not used as a therapeutic or prophylactic agent in vivo, as its effects are small, possibly due to low blood-brain barrier permeability, and complicated by its intrinsic carcinogenicity, In this paper, the development is described of a structure-activity profile for CR by testing a series of analogues of this dye for their ability to inhibit the formation of the protease-resistant prion protein, PrP-res, a molecular marker for the infectious agent, in the scrapie-infected, SMB cell line. It was found that the central benzidine unit in CR, which gives the molecule potential carcinogenicity, can be replaced by other, less toxic moieties and that the sulphonate groups on the core molecule can be replaced by carboxylic acids, which should improve the brain permeability of these compounds. However, detailed dose-response curves were generated for several derivatives and they revealed that, while some compounds showed inhibition of PrP-res accumulation at high concentrations, at low concentrations they actually stimulated levels of PrP-res above control values.

    Original languageEnglish
    Pages (from-to)1155-1164
    Number of pages10
    JournalJournal of General Virology
    Volume81
    Issue number4
    Publication statusPublished - Apr 2000

    Cite this