Screening for high- and low-risk human papillomavirus types in single routine cervical smears by non-isotopic in situ hybridization

C. S. Herrington, G. Troncone, M. F. Evans, J. O'D. McGee

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Routine cervical smears (n = 262) from a Sexually Transmitted Diseases clinic were screened by non-isotopic in situ hybridization (NISH) stratifying human papillomavirus (HPV) infections into HPV6/11 (low risk) and HPV16/18/33 (high risk) categories. Of 188 patients with cytologically normal smears, HPV sequences were demonstrated in 41 %. Of the 128 cases analysed by dual NISH, 16 % contained low risk, 20 % high risk and 5 % both groups. In patients with cytological evidence of wart virus infection (WVI) only, 54 % (n = 50) contained high-risk and 22 % low-risk HPV types. The comparable incidences in CIN1/2 plus WVI (n = 24) were not significantly different: 54 % and 17 %, respectively. Cytological criteria underestimate the prevalance of HPV infection in patients with cytologically normal smears. This represents either 'occult' or 'latent' infection. The identical prevalence of HPVB16/18/33 in WVI only, and CIN1/2 plus WVI, suggests that the cytopathic effect induced by these HPVs may represent one end of a spectrum of morphological change which progresses to cervical intraepithelial neoplasia (CIN).

    Original languageEnglish
    Pages (from-to)71-78
    Number of pages8
    JournalCytopathology : Official Journal of the British Society for Clinical Cytology
    Volume3
    Issue number2
    DOIs
    Publication statusPublished - 1992

    Cite this

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    title = "Screening for high- and low-risk human papillomavirus types in single routine cervical smears by non-isotopic in situ hybridization",
    abstract = "Routine cervical smears (n = 262) from a Sexually Transmitted Diseases clinic were screened by non-isotopic in situ hybridization (NISH) stratifying human papillomavirus (HPV) infections into HPV6/11 (low risk) and HPV16/18/33 (high risk) categories. Of 188 patients with cytologically normal smears, HPV sequences were demonstrated in 41 {\%}. Of the 128 cases analysed by dual NISH, 16 {\%} contained low risk, 20 {\%} high risk and 5 {\%} both groups. In patients with cytological evidence of wart virus infection (WVI) only, 54 {\%} (n = 50) contained high-risk and 22 {\%} low-risk HPV types. The comparable incidences in CIN1/2 plus WVI (n = 24) were not significantly different: 54 {\%} and 17 {\%}, respectively. Cytological criteria underestimate the prevalance of HPV infection in patients with cytologically normal smears. This represents either 'occult' or 'latent' infection. The identical prevalence of HPVB16/18/33 in WVI only, and CIN1/2 plus WVI, suggests that the cytopathic effect induced by these HPVs may represent one end of a spectrum of morphological change which progresses to cervical intraepithelial neoplasia (CIN).",
    author = "Herrington, {C. S.} and G. Troncone and Evans, {M. F.} and McGee, {J. O'D.}",
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    Screening for high- and low-risk human papillomavirus types in single routine cervical smears by non-isotopic in situ hybridization. / Herrington, C. S.; Troncone, G. ; Evans, M. F. ; McGee, J. O'D.

    In: Cytopathology : Official Journal of the British Society for Clinical Cytology, Vol. 3, No. 2, 1992, p. 71-78.

    Research output: Contribution to journalArticle

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    AU - Troncone, G.

    AU - Evans, M. F.

    AU - McGee, J. O'D.

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    AB - Routine cervical smears (n = 262) from a Sexually Transmitted Diseases clinic were screened by non-isotopic in situ hybridization (NISH) stratifying human papillomavirus (HPV) infections into HPV6/11 (low risk) and HPV16/18/33 (high risk) categories. Of 188 patients with cytologically normal smears, HPV sequences were demonstrated in 41 %. Of the 128 cases analysed by dual NISH, 16 % contained low risk, 20 % high risk and 5 % both groups. In patients with cytological evidence of wart virus infection (WVI) only, 54 % (n = 50) contained high-risk and 22 % low-risk HPV types. The comparable incidences in CIN1/2 plus WVI (n = 24) were not significantly different: 54 % and 17 %, respectively. Cytological criteria underestimate the prevalance of HPV infection in patients with cytologically normal smears. This represents either 'occult' or 'latent' infection. The identical prevalence of HPVB16/18/33 in WVI only, and CIN1/2 plus WVI, suggests that the cytopathic effect induced by these HPVs may represent one end of a spectrum of morphological change which progresses to cervical intraepithelial neoplasia (CIN).

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