Secretion of β-lactamase from K. lactis using pEPS1, a convenient episomal vector designed for the secretion of foreign proteins

I. G. Macreadie, L. A. Castelli, A. C. Ward, M. J. R. Stark, A A Azad

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    6 Citations (Scopus)

    Abstract

    A convenient shuttle vector that enables high level secretion of proteins from Kluyveromyces lactis has been developed. The vector, pEPS1, contains a unique cloning site that allows the construction, in a single ligation step, of episomal plasmids capable of directing secretion of foreign gene products from K. lactis. As an example we demonstrate the production of ß-lactamase and determine optimal conditions for its secretion into the culture media.

    Original languageEnglish
    Pages (from-to)213-218
    Number of pages6
    JournalBiotechnology Letters
    Volume15
    Issue number3
    DOIs
    Publication statusPublished - Mar 1993

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