Secretion of β-lactamase from K. lactis using pEPS1, a convenient episomal vector designed for the secretion of foreign proteins

I. G.   Macreadie; L. A.  Castelli; A. C.  Ward; M. J. R.  Stark; A A Azad

doi:10.1007/BF00128307

Secretion of β-lactamase from K. lactis using pEPS₁, a convenient episomal vector designed for the secretion of foreign proteins

I. G. Macreadie
, L. A. Castelli
, A. C. Ward
, M. J. R. Stark
, A A Azad

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A convenient shuttle vector that enables high level secretion of proteins from Kluyveromyces lactis has been developed. The vector, pEPS1, contains a unique cloning site that allows the construction, in a single ligation step, of episomal plasmids capable of directing secretion of foreign gene products from K. lactis. As an example we demonstrate the production of ß-lactamase and determine optimal conditions for its secretion into the culture media.

Original language	English
Pages (from-to)	213-218
Number of pages	6
Journal	Biotechnology Letters
Volume	15
Issue number	3
DOIs	https://doi.org/10.1007/BF00128307
Publication status	Published - Mar 1993

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title = "Secretion of β-lactamase from K. lactis using pEPS1, a convenient episomal vector designed for the secretion of foreign proteins",

abstract = "A convenient shuttle vector that enables high level secretion of proteins from Kluyveromyces lactis has been developed. The vector, pEPS1, contains a unique cloning site that allows the construction, in a single ligation step, of episomal plasmids capable of directing secretion of foreign gene products from K. lactis. As an example we demonstrate the production of {\ss}-lactamase and determine optimal conditions for its secretion into the culture media.",

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AU - Macreadie, I. G.

AU - Castelli, L. A.

AU - Ward, A. C.

AU - Stark, M. J. R.

AU - Azad, A A

PY - 1993/3

Y1 - 1993/3

N2 - A convenient shuttle vector that enables high level secretion of proteins from Kluyveromyces lactis has been developed. The vector, pEPS1, contains a unique cloning site that allows the construction, in a single ligation step, of episomal plasmids capable of directing secretion of foreign gene products from K. lactis. As an example we demonstrate the production of ß-lactamase and determine optimal conditions for its secretion into the culture media.

AB - A convenient shuttle vector that enables high level secretion of proteins from Kluyveromyces lactis has been developed. The vector, pEPS1, contains a unique cloning site that allows the construction, in a single ligation step, of episomal plasmids capable of directing secretion of foreign gene products from K. lactis. As an example we demonstrate the production of ß-lactamase and determine optimal conditions for its secretion into the culture media.

U2 - 10.1007/BF00128307

DO - 10.1007/BF00128307

M3 - Article

SN - 0141-5492

VL - 15

SP - 213

EP - 218

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 3

ER -

Secretion of β-lactamase from K. lactis using pEPS1, a convenient episomal vector designed for the secretion of foreign proteins

Abstract

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Secretion of β-lactamase from K. lactis using pEPS₁, a convenient episomal vector designed for the secretion of foreign proteins