“Seeing” invisible volatile organic compound (VOC) marker of urinary bladder cancer: A development from bench to bedside prototype spectroscopic device

Neha Bhattacharyya, Dipanjan Mukherjee, Soumendra Singh, Ria Ghosh, Saurav Karmakar, Ankita Mallick, Arpita Chattopadhyay, Pulak Mondal, Tapan Mondal, Debasis Bhattacharyya, Asim Kumar Mallick, Ghulam Nabi, Samir Kumar Pal (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

Abstract

Urinary bladder cancer (UBC) is one of the most common cancers and has notoriously high risk of recurrence and mortality across the globe. Current clinical initial diagnostic approaches are either invasive or lacks sensitivity. In this study, an attempt has been made to invent a cost-effective, novel, portable diagnostic device based on the environmental sensitive fluorophores namely Nile Red (NR), Eosin Y (EY) and Rose Bengal (RB). They act as sensing agents for detecting volatile organic compounds (VOC) exclusively present in the urine sample of UBC patients and differentiate the UBC samples from the healthy control group. Upon exposure with a particular group of VOCs, a significant amount of increment in fluorescence intensities of NR, EY and RB were detected and recorded in our indigenously developed “NABIL” device. To check the performance of NABIL, the data collected from the device was compared with the conventional techniques by arranging a clinical trial with 21 healthy controls and 52 UBC patients. With the assistance of our analysis technique based on LabVIEW platform, very high sensitivity and accuracy from healthy controls have been achieved. For UBC patients, it shows impressive diagnostic results. In addition, depending on the sample processing mechanism, NABIL device can also reveal the grade of UBC and prognosis under treatment. Overall, this study contributes a novel, non-invasive, easy-to-use, inexpensive, real-time, accurate method for selectively UBC diagnosis, which can be useful for personalized care/diagnosis and postoperative surveillance, resulting in saving more lives.

Original languageEnglish
Article number114764
Number of pages10
JournalBiosensors and Bioelectronics
Volume218
Early online date4 Oct 2022
DOIs
Publication statusE-pub ahead of print - 4 Oct 2022

Keywords

  • Non-invasive diagnosis
  • Spectroscopic detection
  • Urinary bladder cancer
  • Volatile organic compounds

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