Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore

Susan Chalmers, Stuart T. Caldwell, Caroline Quin, Tracy A. Prime, Andrew M. James, Andrew G. Cairns, Michael P. Murphy, John G. McCarron, Richard C. Hartley

Research output: Contribution to journalArticlepeer-review

80 Citations (Scopus)

Abstract

Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.

Original languageEnglish
Pages (from-to)758-761
Number of pages4
JournalJournal of the American Chemical Society
Volume134
Issue number2
Early online date22 Dec 2011
DOIs
Publication statusPublished - 18 Jan 2012

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