Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore

Susan Chalmers; Stuart T. Caldwell; Caroline Quin; Tracy A. Prime; Andrew M. James; Andrew G. Cairns; Michael P. Murphy; John G. McCarron; Richard C. Hartley

doi:10.1021/ja2077922

Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore

Susan Chalmers, Stuart T. Caldwell, Caroline Quin, Tracy A. Prime, Andrew M. James, Andrew G. Cairns, Michael P. Murphy, John G. McCarron, Richard C. Hartley

Drug Discovery Unit

Research output: Contribution to journal › Article › peer-review

106 Citations (Scopus)

Abstract

Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.

Original language	English
Pages (from-to)	758-761
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	134
Issue number	2
Early online date	22 Dec 2011
DOIs	https://doi.org/10.1021/ja2077922
Publication status	Published - 18 Jan 2012

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja2077922

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title = "Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore",

abstract = "Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.",

author = "Susan Chalmers and Caldwell, \{Stuart T.\} and Caroline Quin and Prime, \{Tracy A.\} and James, \{Andrew M.\} and Cairns, \{Andrew G.\} and Murphy, \{Michael P.\} and McCarron, \{John G.\} and Hartley, \{Richard C.\}",

year = "2012",

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AU - Chalmers, Susan

AU - Caldwell, Stuart T.

AU - Quin, Caroline

AU - Prime, Tracy A.

AU - James, Andrew M.

AU - Cairns, Andrew G.

AU - Murphy, Michael P.

AU - McCarron, John G.

AU - Hartley, Richard C.

PY - 2012/1/18

Y1 - 2012/1/18

N2 - Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.

AB - Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.

UR - https://www.scopus.com/pages/publications/84855960363

U2 - 10.1021/ja2077922

DO - 10.1021/ja2077922

M3 - Article

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EP - 761

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 2

ER -

Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore

Abstract

ASJC Scopus subject areas

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