Self-organization of rat cardiac cells into contractile 3-D cardiac tissue

Keith Baar, Ravi Birla, Marvin O. Boluyt, Gregory H. Borschel, Ellen M. Arruda, Robert G. Dennis

    Research output: Contribution to journalArticle

    97 Citations (Scopus)

    Abstract

    Engineering cardiac tissue without the mechanical restriction of scaffolds has many important scientific applications. Even small functional cardiac tissue constructs could be used to study the effects of gene therapy or various drugs on cardiac tissue formation and contractility. On a larger scale, engineered cardiac muscle could be used to develop replacement tissue for individuals with heart failure. Furthermore, directing neonatal cardiomyocytes to self-organize into contractile cardiac tissue may yield valuable information on essential developmental processes. We describe a method for culturing cardiomyocytes and fibroblasts in such a way as to promote the self-organization of a contractile cardiac muscle organ in culture. We designate these tissue constructs cardioids, as they are similar to cardiac muscle in terms of cell-to-cell connectivity and contractility.
    Original languageEnglish
    Pages (from-to)275-277
    Number of pages3
    JournalFASEB Journal
    Volume19
    Issue number2
    DOIs
    Publication statusPublished - Feb 2005

    Fingerprint

    Rats
    Tissue
    Myocardium
    Muscle
    Cardiac Myocytes
    Organ Culture Techniques
    Gene therapy
    Tissue Engineering
    Genetic Therapy
    Fibroblasts
    Scaffolds
    Heart Failure
    Pharmaceutical Preparations

    Keywords

    • Cardiac muscle
    • Cell physiology
    • Cardiomyocytes
    • Fibroblasts

    Cite this

    Baar, K., Birla, R., Boluyt, M. O., Borschel, G. H., Arruda, E. M., & Dennis, R. G. (2005). Self-organization of rat cardiac cells into contractile 3-D cardiac tissue. FASEB Journal, 19(2), 275-277. https://doi.org/10.1096/fj.04-2034fje
    Baar, Keith ; Birla, Ravi ; Boluyt, Marvin O. ; Borschel, Gregory H. ; Arruda, Ellen M. ; Dennis, Robert G. / Self-organization of rat cardiac cells into contractile 3-D cardiac tissue. In: FASEB Journal. 2005 ; Vol. 19, No. 2. pp. 275-277.
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    Baar, K, Birla, R, Boluyt, MO, Borschel, GH, Arruda, EM & Dennis, RG 2005, 'Self-organization of rat cardiac cells into contractile 3-D cardiac tissue', FASEB Journal, vol. 19, no. 2, pp. 275-277. https://doi.org/10.1096/fj.04-2034fje

    Self-organization of rat cardiac cells into contractile 3-D cardiac tissue. / Baar, Keith; Birla, Ravi; Boluyt, Marvin O.; Borschel, Gregory H.; Arruda, Ellen M.; Dennis, Robert G.

    In: FASEB Journal, Vol. 19, No. 2, 02.2005, p. 275-277.

    Research output: Contribution to journalArticle

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    AU - Dennis, Robert G.

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    Baar K, Birla R, Boluyt MO, Borschel GH, Arruda EM, Dennis RG. Self-organization of rat cardiac cells into contractile 3-D cardiac tissue. FASEB Journal. 2005 Feb;19(2):275-277. https://doi.org/10.1096/fj.04-2034fje