Semen Analysis and Sperm Function Tests

Hannah Williams, Sarah Martins Da Silva

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review


Semen analysis is a fundamental part of male fertility investigation. Samples are produced by masturbation, ideally in a private room nearby to the andrology laboratory or submitted within one hour if produced off-site. The ejaculate should be collected in a wide-mouthed, clean, glass or plastic container, from a batch confirmed to be non-toxic to spermatozoa. Patients should be given clear instructions about collection of their sample. To ensure consistency and reliable interpretation of results, there should be a minimum of two days and maximum of seven days abstinence (1), and the complete sample should be collected. Loss of the first portion of the ejaculate, which mainly comprises sperm-rich prostatic fluid, may significantly affect sperm count and concentration.

Original languageEnglish
Title of host publicationSubfertility, Reproductive Endocrinology and Assisted Reproduction
EditorsJane A. Stewart
PublisherCambridge University Press
Number of pages7
ISBN (Electronic)9781316488294
Publication statusPublished - 2019


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