Separation and quantitation of the polyamine biosynthesis inhibitor d,l-α-difluoromethylarginine and other guanidine-containing compounds by high-performance liquid chromatography

Karl J. Hunter, Alan H. Fairlamb

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    The arginine decarboxylase inhibitor difluoromethylarginine (DFMA) is an important tool in the study of polyamine metabolism, particularly with respect to the human pathogen Trypanosoma cruzi. This paper demonstrates a unique method for the detection and quantitation of intracellular DFMA using the fluorogenic agent 9,10-phenanthrenequinone. After separation of cell extracts by HPLC, DFMA can be accurately and reproducibly quantified with a lower sensitivity limit of 0.1 nmol by this simple fluorometric method. This assay can also be used to detect other guanidine-containing compounds such as arginine, agmatine, creatinine, and hirudonine, but not substituted guanidines such as aminoguanidine and creatine, or the structurally related amidines such as benzamidine and pentamidine.

    Original languageEnglish
    Pages (from-to)281-285
    Number of pages5
    JournalAnalytical Biochemistry
    Volume190
    Issue number2
    DOIs
    Publication statusPublished - 1 Nov 1990

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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