TY - JOUR
T1 - Separation and quantitation of the polyamine biosynthesis inhibitor d,l-α-difluoromethylarginine and other guanidine-containing compounds by high-performance liquid chromatography
AU - Hunter, Karl J.
AU - Fairlamb, Alan H.
PY - 1990/11/1
Y1 - 1990/11/1
N2 - The arginine decarboxylase inhibitor difluoromethylarginine (DFMA) is an important tool in the study of polyamine metabolism, particularly with respect to the human pathogen Trypanosoma cruzi. This paper demonstrates a unique method for the detection and quantitation of intracellular DFMA using the fluorogenic agent 9,10-phenanthrenequinone. After separation of cell extracts by HPLC, DFMA can be accurately and reproducibly quantified with a lower sensitivity limit of 0.1 nmol by this simple fluorometric method. This assay can also be used to detect other guanidine-containing compounds such as arginine, agmatine, creatinine, and hirudonine, but not substituted guanidines such as aminoguanidine and creatine, or the structurally related amidines such as benzamidine and pentamidine.
AB - The arginine decarboxylase inhibitor difluoromethylarginine (DFMA) is an important tool in the study of polyamine metabolism, particularly with respect to the human pathogen Trypanosoma cruzi. This paper demonstrates a unique method for the detection and quantitation of intracellular DFMA using the fluorogenic agent 9,10-phenanthrenequinone. After separation of cell extracts by HPLC, DFMA can be accurately and reproducibly quantified with a lower sensitivity limit of 0.1 nmol by this simple fluorometric method. This assay can also be used to detect other guanidine-containing compounds such as arginine, agmatine, creatinine, and hirudonine, but not substituted guanidines such as aminoguanidine and creatine, or the structurally related amidines such as benzamidine and pentamidine.
UR - http://www.scopus.com/inward/record.url?scp=0025114465&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(90)90194-E
DO - 10.1016/0003-2697(90)90194-E
M3 - Article
C2 - 2291471
AN - SCOPUS:0025114465
SN - 0003-2697
VL - 190
SP - 281
EP - 285
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -