Separation and quantitation of the polyamine biosynthesis inhibitor d,l-α-difluoromethylarginine and other guanidine-containing compounds by high-performance liquid chromatography

Karl J. Hunter; Alan H. Fairlamb

doi:10.1016/0003-2697(90)90194-E

Separation and quantitation of the polyamine biosynthesis inhibitor d,l-α-difluoromethylarginine and other guanidine-containing compounds by high-performance liquid chromatography

Karl J. Hunter
, Alan H. Fairlamb

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The arginine decarboxylase inhibitor difluoromethylarginine (DFMA) is an important tool in the study of polyamine metabolism, particularly with respect to the human pathogen Trypanosoma cruzi. This paper demonstrates a unique method for the detection and quantitation of intracellular DFMA using the fluorogenic agent 9,10-phenanthrenequinone. After separation of cell extracts by HPLC, DFMA can be accurately and reproducibly quantified with a lower sensitivity limit of 0.1 nmol by this simple fluorometric method. This assay can also be used to detect other guanidine-containing compounds such as arginine, agmatine, creatinine, and hirudonine, but not substituted guanidines such as aminoguanidine and creatine, or the structurally related amidines such as benzamidine and pentamidine.

Original language	English
Pages (from-to)	281-285
Number of pages	5
Journal	Analytical Biochemistry
Volume	190
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(90)90194-E
Publication status	Published - 1 Nov 1990

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Separation and quantitation of the polyamine biosynthesis inhibitor d,l-α-difluoromethylarginine and other guanidine-containing compounds by high-performance liquid chromatography",

abstract = "The arginine decarboxylase inhibitor difluoromethylarginine (DFMA) is an important tool in the study of polyamine metabolism, particularly with respect to the human pathogen Trypanosoma cruzi. This paper demonstrates a unique method for the detection and quantitation of intracellular DFMA using the fluorogenic agent 9,10-phenanthrenequinone. After separation of cell extracts by HPLC, DFMA can be accurately and reproducibly quantified with a lower sensitivity limit of 0.1 nmol by this simple fluorometric method. This assay can also be used to detect other guanidine-containing compounds such as arginine, agmatine, creatinine, and hirudonine, but not substituted guanidines such as aminoguanidine and creatine, or the structurally related amidines such as benzamidine and pentamidine.",

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Y1 - 1990/11/1

N2 - The arginine decarboxylase inhibitor difluoromethylarginine (DFMA) is an important tool in the study of polyamine metabolism, particularly with respect to the human pathogen Trypanosoma cruzi. This paper demonstrates a unique method for the detection and quantitation of intracellular DFMA using the fluorogenic agent 9,10-phenanthrenequinone. After separation of cell extracts by HPLC, DFMA can be accurately and reproducibly quantified with a lower sensitivity limit of 0.1 nmol by this simple fluorometric method. This assay can also be used to detect other guanidine-containing compounds such as arginine, agmatine, creatinine, and hirudonine, but not substituted guanidines such as aminoguanidine and creatine, or the structurally related amidines such as benzamidine and pentamidine.

AB - The arginine decarboxylase inhibitor difluoromethylarginine (DFMA) is an important tool in the study of polyamine metabolism, particularly with respect to the human pathogen Trypanosoma cruzi. This paper demonstrates a unique method for the detection and quantitation of intracellular DFMA using the fluorogenic agent 9,10-phenanthrenequinone. After separation of cell extracts by HPLC, DFMA can be accurately and reproducibly quantified with a lower sensitivity limit of 0.1 nmol by this simple fluorometric method. This assay can also be used to detect other guanidine-containing compounds such as arginine, agmatine, creatinine, and hirudonine, but not substituted guanidines such as aminoguanidine and creatine, or the structurally related amidines such as benzamidine and pentamidine.

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DO - 10.1016/0003-2697(90)90194-E

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ER -

Separation and quantitation of the polyamine biosynthesis inhibitor d,l-α-difluoromethylarginine and other guanidine-containing compounds by high-performance liquid chromatography

Abstract

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