Serine 15 phosphorylation stimulates p53 transactivation but does not directly influence interaction with HDM2

Nicolas Dumaz, David W. Meek

    Research output: Contribution to journalArticle

    381 Citations (Scopus)

    Abstract

    The p53 tumour suppressor protein is a labile transcription factor that is activated and stabilized in response to a wide range of cellular stresses, through a mechanism involving disruption of its interaction with MDM2, a negative regulatory partner. Induction of p53 by DNA damage additionally involves a series of phosphorylation and acetylation modifications, some of which are thought to regulate MDM2 binding. Here we report the effects of introducing mutations at several known or putative N-terminal phosphorylation sites on the transactivation function of p53. These studies highlight phosphorylation of Ser15, a key phosphorylation target during the p53 activation process, as being critical for p53-dependent transactivation. Biochemical data indicate that the mechanism by which phosphorylation of Ser15 stimulates p53-dependent transactivation occurs through increased binding to the p300 coactivator protein. The data also indicate that Ser15-dependent regulation of transactivation is independent of any involvement in modulating MDM2 binding, and that Ser15 phosphorylation alone is not sufficient to block the p53-MDM2 interaction.
    Original languageEnglish
    Pages (from-to)7002-7010
    Number of pages9
    JournalEMBO Journal
    Volume18
    Issue number24
    DOIs
    Publication statusPublished - 15 Dec 1999

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