SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids

TY - JOUR

T1 - SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids

AU - Skinner, William H.

AU - Robinson, Nicola

AU - Hardisty, Gareth R.

AU - Fleming, Holly

AU - Geddis, Ailsa

AU - Bradley, Mark

AU - Gray, Robert D.

AU - Campbell, Colin J.

N1 - Funding Information: This work was supported by the EPSRC CDT in Intelligent Sensing and Measurement (EP/L016753/1) and EPSRC CDT in Optical Medical Imaging (EP/L016559/1). Copyright: © The Royal Society of Chemistry 2023.

PY - 2023/3/18

Y1 - 2023/3/18

N2 - Patient derived organoids have the potential to improve the physiological relevance of in vitro disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM.

AB - Patient derived organoids have the potential to improve the physiological relevance of in vitro disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM.

UR - https://www.scopus.com/pages/publications/85149067570

U2 - 10.1039/d2cc06582g

DO - 10.1039/d2cc06582g

M3 - Article

C2 - 36815668

SN - 1359-7345

VL - 59

SP - 3249

EP - 3252

JO - Chemical Communications

JF - Chemical Communications

IS - 22

ER -