Sex-specific constitutive expression of the pre-neoplastic marker glutathione S-transferase, YfYf, in mouse liver

L I McLellan, J D Hayes

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    Hepatic glutathione S-transferase isoenzyme content has been investigated in both sexes of three inbred strains of mice (DBA/2, C3H/He, C57BL6). A polypeptide (Mr 24,800), which is immunologically related to Yf purified from rat lung, was found to be expressed as a major form in all male mouse livers but represented only a minor enzyme form in female mouse liver. Glutathione S-transferases comprising subunits with molecular masses of 25,800 (Ya) or 26,400 (Yb) were present in males and females of the three strains under investigation. Cytosolic isoenzymes from all strains and sexes were purified to apparent homogeneity and no significant inter-strain differences in the properties of the individual forms were observed. In addition, no differences were detected in the microsomal glutathione S-transferase content of the different strains or sexes.

    Original languageEnglish
    Pages (from-to)399-406
    Number of pages8
    JournalBiochemical Journal
    Volume245
    Issue number2
    DOIs
    Publication statusPublished - 15 Jul 1987

    Keywords

    • Animals
    • Chromatography, High Pressure Liquid
    • Electrophoresis, Polyacrylamide Gel
    • Ethylmaleimide/pharmacology
    • Female
    • Glutathione Transferase/metabolism
    • Immunoelectrophoresis
    • Isoenzymes/metabolism
    • Liver/enzymology
    • Male
    • Mice
    • Mice, Inbred Strains
    • Microsomes, Liver/drug effects
    • Sex Factors
    • Substrate Specificity

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