Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host-cell recognition

Biswajit Khatua; Angana Ghoshal; Kaushik Bhattacharya; Chandan Mandal; Bibhuti Saha; Paul R. Crocker; Chitra Mandal; Sandro Sonnino

doi:10.1016/j.febslet.2009.11.087

Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host-cell recognition

Biswajit Khatua, Angana Ghoshal, Kaushik Bhattacharya, Chandan Mandal, Bibhuti Saha, Paul R. Crocker, Chitra Mandal, Sandro Sonnino (Editor)

Research output: Contribution to journal › Article › peer-review

64 Citations (Scopus)

Abstract

The opportunism of Pseudomonas aeruginosa (PA) in immunocompromised hosts prompted us to explore the potential role of sialic acids (Sia) in this phenomenon. Culture of PA in the presence of exogenous Sia resulted in linkage-specific incorporation of Sia which was associated with decreased complement deposition on the bacteria. Sia acquired by PA mediated enhanced binding of bacteria to recombinant-CHO cells expressing human siglec-7 or siglec-9, as well as to human NK-cells and monocytes naturally expressing these siglecs. Therefore, Sia may be acquired by PA in the host and contribute to bacterial pathogenicity and host-cell interactions via reduction of complement deposition and siglec-dependent recognition. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	555-561
Number of pages	7
Journal	FEBS Letters
Volume	584
Issue number	3
DOIs	https://doi.org/10.1016/j.febslet.2009.11.087
Publication status	Published - 5 Feb 2010

Keywords

Complement
Sialic acid
Siglec specific recognition
Visceral leishmaniasis
9-O-acetyl sialic acid
Pseudomonas aeruginosa
Binding
Lipopolysaccharide
Leukocytes

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10.1016/j.febslet.2009.11.087

Aref#d: 19149. Role of Siglecs in Disease (Senior Research Fellowship)
Crocker, P.
Wellcome Trust
1/11/07 → 30/04/13
Project: Research

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@article{3a5e65f1b3f24983b506868ee1c0710a,

title = "Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host-cell recognition",

abstract = "The opportunism of Pseudomonas aeruginosa (PA) in immunocompromised hosts prompted us to explore the potential role of sialic acids (Sia) in this phenomenon. Culture of PA in the presence of exogenous Sia resulted in linkage-specific incorporation of Sia which was associated with decreased complement deposition on the bacteria. Sia acquired by PA mediated enhanced binding of bacteria to recombinant-CHO cells expressing human siglec-7 or siglec-9, as well as to human NK-cells and monocytes naturally expressing these siglecs. Therefore, Sia may be acquired by PA in the host and contribute to bacterial pathogenicity and host-cell interactions via reduction of complement deposition and siglec-dependent recognition. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.",

keywords = "Complement, Sialic acid, Siglec specific recognition, Visceral leishmaniasis, 9-O-acetyl sialic acid, Pseudomonas aeruginosa, Binding, Lipopolysaccharide, Leukocytes",

author = "Biswajit Khatua and Angana Ghoshal and Kaushik Bhattacharya and Chandan Mandal and Bibhuti Saha and Crocker, {Paul R.} and Chitra Mandal and Sandro Sonnino",

year = "2010",

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doi = "10.1016/j.febslet.2009.11.087",

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T1 - Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host-cell recognition

AU - Khatua, Biswajit

AU - Ghoshal, Angana

AU - Bhattacharya, Kaushik

AU - Mandal, Chandan

AU - Saha, Bibhuti

AU - Crocker, Paul R.

AU - Mandal, Chitra

A2 - Sonnino, Sandro

PY - 2010/2/5

Y1 - 2010/2/5

N2 - The opportunism of Pseudomonas aeruginosa (PA) in immunocompromised hosts prompted us to explore the potential role of sialic acids (Sia) in this phenomenon. Culture of PA in the presence of exogenous Sia resulted in linkage-specific incorporation of Sia which was associated with decreased complement deposition on the bacteria. Sia acquired by PA mediated enhanced binding of bacteria to recombinant-CHO cells expressing human siglec-7 or siglec-9, as well as to human NK-cells and monocytes naturally expressing these siglecs. Therefore, Sia may be acquired by PA in the host and contribute to bacterial pathogenicity and host-cell interactions via reduction of complement deposition and siglec-dependent recognition. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

AB - The opportunism of Pseudomonas aeruginosa (PA) in immunocompromised hosts prompted us to explore the potential role of sialic acids (Sia) in this phenomenon. Culture of PA in the presence of exogenous Sia resulted in linkage-specific incorporation of Sia which was associated with decreased complement deposition on the bacteria. Sia acquired by PA mediated enhanced binding of bacteria to recombinant-CHO cells expressing human siglec-7 or siglec-9, as well as to human NK-cells and monocytes naturally expressing these siglecs. Therefore, Sia may be acquired by PA in the host and contribute to bacterial pathogenicity and host-cell interactions via reduction of complement deposition and siglec-dependent recognition. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

KW - Complement

KW - Sialic acid

KW - Siglec specific recognition

KW - Visceral leishmaniasis

KW - 9-O-acetyl sialic acid

KW - Pseudomonas aeruginosa

KW - Binding

KW - Lipopolysaccharide

KW - Leukocytes

U2 - 10.1016/j.febslet.2009.11.087

DO - 10.1016/j.febslet.2009.11.087

M3 - Article

C2 - 19945458

SN - 0014-5793

VL - 584

SP - 555

EP - 561

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host-cell recognition

Abstract

Keywords

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Projects

Aref#d: 19149. Role of Siglecs in Disease (Senior Research Fellowship)

Cite this