Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host-cell recognition

Biswajit Khatua, Angana Ghoshal, Kaushik Bhattacharya, Chandan Mandal, Bibhuti Saha, Paul R. Crocker, Chitra Mandal, Sandro Sonnino (Editor)

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    Abstract

    The opportunism of Pseudomonas aeruginosa (PA) in immunocompromised hosts prompted us to explore the potential role of sialic acids (Sia) in this phenomenon. Culture of PA in the presence of exogenous Sia resulted in linkage-specific incorporation of Sia which was associated with decreased complement deposition on the bacteria. Sia acquired by PA mediated enhanced binding of bacteria to recombinant-CHO cells expressing human siglec-7 or siglec-9, as well as to human NK-cells and monocytes naturally expressing these siglecs. Therefore, Sia may be acquired by PA in the host and contribute to bacterial pathogenicity and host-cell interactions via reduction of complement deposition and siglec-dependent recognition. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)555-561
    Number of pages7
    JournalFEBS Letters
    Volume584
    Issue number3
    DOIs
    Publication statusPublished - 5 Feb 2010

    Keywords

    • Complement
    • Sialic acid
    • Siglec specific recognition
    • Visceral leishmaniasis
    • 9-O-acetyl sialic acid
    • Pseudomonas aeruginosa
    • Binding
    • Lipopolysaccharide
    • Leukocytes

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