Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host-cell recognition

Biswajit Khatua, Angana Ghoshal, Kaushik Bhattacharya, Chandan Mandal, Bibhuti Saha, Paul R. Crocker, Chitra Mandal, Sandro Sonnino (Editor)

    Research output: Contribution to journalArticle

    40 Citations (Scopus)

    Abstract

    The opportunism of Pseudomonas aeruginosa (PA) in immunocompromised hosts prompted us to explore the potential role of sialic acids (Sia) in this phenomenon. Culture of PA in the presence of exogenous Sia resulted in linkage-specific incorporation of Sia which was associated with decreased complement deposition on the bacteria. Sia acquired by PA mediated enhanced binding of bacteria to recombinant-CHO cells expressing human siglec-7 or siglec-9, as well as to human NK-cells and monocytes naturally expressing these siglecs. Therefore, Sia may be acquired by PA in the host and contribute to bacterial pathogenicity and host-cell interactions via reduction of complement deposition and siglec-dependent recognition. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)555-561
    Number of pages7
    JournalFEBS Letters
    Volume584
    Issue number3
    DOIs
    Publication statusPublished - 5 Feb 2010

    Keywords

    • Complement
    • Sialic acid
    • Siglec specific recognition
    • Visceral leishmaniasis
    • 9-O-acetyl sialic acid
    • Pseudomonas aeruginosa
    • Binding
    • Lipopolysaccharide
    • Leukocytes

    Cite this

    Khatua, Biswajit ; Ghoshal, Angana ; Bhattacharya, Kaushik ; Mandal, Chandan ; Saha, Bibhuti ; Crocker, Paul R. ; Mandal, Chitra ; Sonnino, Sandro (Editor). / Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host-cell recognition. In: FEBS Letters. 2010 ; Vol. 584, No. 3. pp. 555-561.
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    abstract = "The opportunism of Pseudomonas aeruginosa (PA) in immunocompromised hosts prompted us to explore the potential role of sialic acids (Sia) in this phenomenon. Culture of PA in the presence of exogenous Sia resulted in linkage-specific incorporation of Sia which was associated with decreased complement deposition on the bacteria. Sia acquired by PA mediated enhanced binding of bacteria to recombinant-CHO cells expressing human siglec-7 or siglec-9, as well as to human NK-cells and monocytes naturally expressing these siglecs. Therefore, Sia may be acquired by PA in the host and contribute to bacterial pathogenicity and host-cell interactions via reduction of complement deposition and siglec-dependent recognition. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.",
    keywords = "Complement, Sialic acid, Siglec specific recognition, Visceral leishmaniasis, 9-O-acetyl sialic acid, Pseudomonas aeruginosa, Binding, Lipopolysaccharide, Leukocytes",
    author = "Biswajit Khatua and Angana Ghoshal and Kaushik Bhattacharya and Chandan Mandal and Bibhuti Saha and Crocker, {Paul R.} and Chitra Mandal and Sandro Sonnino",
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    Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host-cell recognition. / Khatua, Biswajit; Ghoshal, Angana; Bhattacharya, Kaushik; Mandal, Chandan; Saha, Bibhuti; Crocker, Paul R.; Mandal, Chitra; Sonnino, Sandro (Editor).

    In: FEBS Letters, Vol. 584, No. 3, 05.02.2010, p. 555-561.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host-cell recognition

    AU - Khatua, Biswajit

    AU - Ghoshal, Angana

    AU - Bhattacharya, Kaushik

    AU - Mandal, Chandan

    AU - Saha, Bibhuti

    AU - Crocker, Paul R.

    AU - Mandal, Chitra

    A2 - Sonnino, Sandro

    PY - 2010/2/5

    Y1 - 2010/2/5

    N2 - The opportunism of Pseudomonas aeruginosa (PA) in immunocompromised hosts prompted us to explore the potential role of sialic acids (Sia) in this phenomenon. Culture of PA in the presence of exogenous Sia resulted in linkage-specific incorporation of Sia which was associated with decreased complement deposition on the bacteria. Sia acquired by PA mediated enhanced binding of bacteria to recombinant-CHO cells expressing human siglec-7 or siglec-9, as well as to human NK-cells and monocytes naturally expressing these siglecs. Therefore, Sia may be acquired by PA in the host and contribute to bacterial pathogenicity and host-cell interactions via reduction of complement deposition and siglec-dependent recognition. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    AB - The opportunism of Pseudomonas aeruginosa (PA) in immunocompromised hosts prompted us to explore the potential role of sialic acids (Sia) in this phenomenon. Culture of PA in the presence of exogenous Sia resulted in linkage-specific incorporation of Sia which was associated with decreased complement deposition on the bacteria. Sia acquired by PA mediated enhanced binding of bacteria to recombinant-CHO cells expressing human siglec-7 or siglec-9, as well as to human NK-cells and monocytes naturally expressing these siglecs. Therefore, Sia may be acquired by PA in the host and contribute to bacterial pathogenicity and host-cell interactions via reduction of complement deposition and siglec-dependent recognition. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    KW - Complement

    KW - Sialic acid

    KW - Siglec specific recognition

    KW - Visceral leishmaniasis

    KW - 9-O-acetyl sialic acid

    KW - Pseudomonas aeruginosa

    KW - Binding

    KW - Lipopolysaccharide

    KW - Leukocytes

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