Sialoadhesin Promotes Rapid Proinflammatory and Type I IFN Responses to a Sialylated Pathogen, Campylobacter jejuni

Mariliis Klaas, Cornelia Oetke, Leanne E Lewis, Lars P Erwig, Astrid P Heikema, Alistair Easton, Hugh J Willison, Paul R Crocker (Lead / Corresponding author)

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    Abstract

    Sialoadhesin (Sn) is a macrophage (M)-restricted receptor that recognizes sialylated ligands on host cells and pathogens. Although Sn is thought to be important in cellular interactions of Ms with cells of the immune system, the functional consequences of pathogen engagement by Sn are unclear. As a model system, we have investigated the role of Sn in M interactions with heat-killed Campylobacter jejuni expressing a GD1a-like, sialylated glycan. Compared to Sn-expressing bone marrow-derived macrophages (BMDM) from wild-type mice, BMDM from mice either deficient in Sn or expressing a non-glycan-binding form of Sn showed greatly reduced phagocytosis of sialylated C. jejuni. This was accompanied by a strong reduction in MyD88-dependent secretion of TNF-a, IL-6, IL-12, and IL-10. In vivo studies demonstrated that functional Sn was required for rapid TNF-a and IFN-ß responses to i.v.-injected sialylated C. jejuni. Bacteria were captured within minutes after i.v. injection and were associated with Ms in both liver and spleen. In the spleen, IFN-ß-reactive cells were localized to Sn(+) Ms and other cells in the red pulp and marginal zone. Together, these studies demonstrate that Sn plays a key role in capturing sialylated pathogens and promoting rapid proinflammatory cytokine and type I IFN responses.
    Original languageEnglish
    Pages (from-to)2414-22
    Number of pages9
    JournalJournal of Immunology
    Volume189
    Issue number5
    DOIs
    Publication statusPublished - 1 Sep 2012

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    Sialic Acid Binding Ig-like Lectin 1
    Campylobacter jejuni
    Macrophages
    Spleen
    Interleukin-12
    Phagocytosis
    Interleukin-10
    Polysaccharides
    Immune System

    Cite this

    Klaas, Mariliis ; Oetke, Cornelia ; Lewis, Leanne E ; Erwig, Lars P ; Heikema, Astrid P ; Easton, Alistair ; Willison, Hugh J ; Crocker, Paul R. / Sialoadhesin Promotes Rapid Proinflammatory and Type I IFN Responses to a Sialylated Pathogen, Campylobacter jejuni. In: Journal of Immunology. 2012 ; Vol. 189, No. 5. pp. 2414-22.
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    abstract = "Sialoadhesin (Sn) is a macrophage (M)-restricted receptor that recognizes sialylated ligands on host cells and pathogens. Although Sn is thought to be important in cellular interactions of Ms with cells of the immune system, the functional consequences of pathogen engagement by Sn are unclear. As a model system, we have investigated the role of Sn in M interactions with heat-killed Campylobacter jejuni expressing a GD1a-like, sialylated glycan. Compared to Sn-expressing bone marrow-derived macrophages (BMDM) from wild-type mice, BMDM from mice either deficient in Sn or expressing a non-glycan-binding form of Sn showed greatly reduced phagocytosis of sialylated C. jejuni. This was accompanied by a strong reduction in MyD88-dependent secretion of TNF-a, IL-6, IL-12, and IL-10. In vivo studies demonstrated that functional Sn was required for rapid TNF-a and IFN-{\ss} responses to i.v.-injected sialylated C. jejuni. Bacteria were captured within minutes after i.v. injection and were associated with Ms in both liver and spleen. In the spleen, IFN-{\ss}-reactive cells were localized to Sn(+) Ms and other cells in the red pulp and marginal zone. Together, these studies demonstrate that Sn plays a key role in capturing sialylated pathogens and promoting rapid proinflammatory cytokine and type I IFN responses.",
    author = "Mariliis Klaas and Cornelia Oetke and Lewis, {Leanne E} and Erwig, {Lars P} and Heikema, {Astrid P} and Alistair Easton and Willison, {Hugh J} and Crocker, {Paul R}",
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    Sialoadhesin Promotes Rapid Proinflammatory and Type I IFN Responses to a Sialylated Pathogen, Campylobacter jejuni. / Klaas, Mariliis; Oetke, Cornelia; Lewis, Leanne E; Erwig, Lars P; Heikema, Astrid P; Easton, Alistair; Willison, Hugh J; Crocker, Paul R (Lead / Corresponding author).

    In: Journal of Immunology, Vol. 189, No. 5, 01.09.2012, p. 2414-22.

    Research output: Contribution to journalArticle

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    AU - Klaas, Mariliis

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    AU - Lewis, Leanne E

    AU - Erwig, Lars P

    AU - Heikema, Astrid P

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