Siglec-G is a B1 cell-inhibitory receptor that controls expansion and calcium signaling of the B1 cell population

Anja Hoffmann, Sheena Kerr, Julia Jellusova, Jiquan Zhang, Florian Weisel, Ute Wellmann, Thomas H. Winkler, Burkhard Kneitz, Paul R. Crocker, Lars Nitschke (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    122 Citations (Scopus)

    Abstract

    B1 cells are an important cell population for the production of natural antibodies and for antibacterial immunoglobulin responses. Here we identified the mouse protein Siglec-G as a B1 cell inhibitory receptor. Siglec-G was expressed in a B cell restricted way, with large amounts present in B1 cells. When overexpressed, Siglec-G inhibited B cell receptor-mediated calcium signaling. Siglec-G-deficient mice had massive expansion of the B1a cell population, which began early in development and was B cell intrinsic. Siglec-G- deficient mice had higher titers of natural IgM antibodies but not a higher penetrance of IgG autoantibodies. Siglec-G-deficient B1 cells showed a strongly enhanced calcium signaling. Our results demonstrate that Siglec-G-dependent negative regulation exists in B1 cells, which may explain the naturally muted signaling response of B1 cells.

    Original languageEnglish
    Pages (from-to)694-704
    Number of pages10
    JournalNature Immunology
    Volume8
    Issue number7
    DOIs
    Publication statusPublished - Jul 2007

    Keywords

    • GENE
    • PROTEIN-TYROSINE-PHOSPHATASE
    • MARGINAL ZONE
    • CD22-DEFICIENT MICE
    • LIFE-SPAN
    • CD22
    • B-1 CELLS
    • MONOCLONAL-ANTIBODIES
    • IMMUNE-SYSTEM
    • NEGATIVE REGULATION

    Cite this

    Hoffmann, A., Kerr, S., Jellusova, J., Zhang, J., Weisel, F., Wellmann, U., Winkler, T. H., Kneitz, B., Crocker, P. R., & Nitschke, L. (2007). Siglec-G is a B1 cell-inhibitory receptor that controls expansion and calcium signaling of the B1 cell population. Nature Immunology, 8(7), 694-704. https://doi.org/10.1038/ni1480