SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis

James W. Opzoomer; Rhianna O'Sullivan; Jahangir Sufi; Ralitsa Madsen; Xiao Qin; Ewa Basiarz; Christopher J. Tape

doi:10.1101/2024.02.23.581433

SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis

James W. Opzoomer, Rhianna O'Sullivan, Jahangir Sufi, Ralitsa Madsen, Xiao Qin, Ewa Basiarz, Christopher J. Tape (Lead / Corresponding author)

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Abstract

We present SIGNAL-seq (Split-pool Indexing siG-Nalling AnaLysis by sequencing): a multiplexed splitpool combinatorial barcoding method that simultaneously measures RNA and post-translational modifications (PTMs) in fixed single cells from 3D models. SIGNAL-seq PTM measurements are equivalent to mass cytometry and RNA gene detection is analogous to split-pool barcoding scRNA-seq. By measuring both mRNA ligand-receptor pairs and PTMs in single cells, SIGNAL-seq can simultaneously uncover inter- and intra-cellular regulation of tumour microenvironment plasticity.

Original language	English
Publisher	BioRxiv
Pages	1-21
Number of pages	21
DOIs	https://doi.org/10.1101/2024.02.23.581433
Publication status	Published - 11 Mar 2024

Keywords

Cancer biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1101/2024.02.23.581433Licence: CC BY-NC

PreprintSubmitted manuscript, 7.57 MBLicence: CC BY-NC

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AU - Madsen, Ralitsa

AU - Qin, Xiao

AU - Basiarz, Ewa

AU - Tape, Christopher J.

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SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis

Abstract

Keywords

UN SDGs

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