TY - JOUR
T1 - Signal transduction by CD28 costimulatory receptor on T cells
T2 - B7-1 and B7-2 regulation of tyrosine kinase adaptor molecules
AU - Nunès, Jacques A.
AU - Truneh, Alemseged
AU - Olive, Daniel
AU - Cantrell, Doreen A.
PY - 1996/1/19
Y1 - 1996/1/19
N2 - This study compares the biochemical responses in T cells activated with the CD28 ligands B7-1 and B7-2. The patterns of tyrosine phosphorylation induced in T cells by these two CD28 ligands are identical, but clearly different from the tyrosine phosphorylation induced by the T cell receptor (TCR). The TCR regulates protein complexes mediated by the adapter Grb2 both in vivo and in vitro. In contrast, there is no apparent regulation of in vivo Grb2 complexes in response to B7-1 or B7-2. Rather, B7-1 and B7-2 both induce tyrosine phosphorylation of a different adapter protein, p62. The regulation of p62 is a unique CD28 response that is not shared with the TCR. These data indicate that B7-1 and B7-2 induce identical tyrosine kinase signal transduction pathways. The data show also that the TCR and CD28 couple to different adapter proteins, which could explain the divergence of TCR and CD28 signal transduction pathways during T cell activation.
AB - This study compares the biochemical responses in T cells activated with the CD28 ligands B7-1 and B7-2. The patterns of tyrosine phosphorylation induced in T cells by these two CD28 ligands are identical, but clearly different from the tyrosine phosphorylation induced by the T cell receptor (TCR). The TCR regulates protein complexes mediated by the adapter Grb2 both in vivo and in vitro. In contrast, there is no apparent regulation of in vivo Grb2 complexes in response to B7-1 or B7-2. Rather, B7-1 and B7-2 both induce tyrosine phosphorylation of a different adapter protein, p62. The regulation of p62 is a unique CD28 response that is not shared with the TCR. These data indicate that B7-1 and B7-2 induce identical tyrosine kinase signal transduction pathways. The data show also that the TCR and CD28 couple to different adapter proteins, which could explain the divergence of TCR and CD28 signal transduction pathways during T cell activation.
UR - http://www.scopus.com/inward/record.url?scp=0030060353&partnerID=8YFLogxK
U2 - 10.1074/jbc.271.3.1591
DO - 10.1074/jbc.271.3.1591
M3 - Article
C2 - 8576157
AN - SCOPUS:0030060353
SN - 0021-9258
VL - 271
SP - 1591
EP - 1598
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 3
ER -