SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

Alistair Langlands, Hamze Beati, H- Arno J Müller (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

The fruit fly Drosophila melanogaster represents a classic genetic model organism that is amenable to a plethora of comprehensive analyses including proteomics. SILAC-based quantitative proteomics is a powerful method to investigate the translational and posttranslational regulation ongoing in cells, tissues, organs, and whole organisms. Here we describe a protocol for routine SILAC labeling of Drosophila adults within one generation to produce embryos with a labeling efficiency of over 92%. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.

Original languageEnglish
Title of host publicationSILAC
Subtitle of host publicationMethods and Protocols
EditorsJose L. Luque-Garcia
Place of PublicationNew York
PublisherHumana Press
Pages187-198
Number of pages12
Edition1
ISBN (Electronic)9781071628638
ISBN (Print)9781071628621
DOIs
Publication statusE-pub ahead of print - 13 Nov 2022

Publication series

NameMethods in Molecular Biology
Volume2603
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Animals
  • Proteomics/methods
  • Isotope Labeling/methods
  • Drosophila melanogaster/genetics
  • Drosophila
  • Protein Processing, Post-Translational
  • Posttranslational modification
  • Embryo
  • Proteomics
  • Development
  • SILAC

Fingerprint

Dive into the research topics of 'SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos'. Together they form a unique fingerprint.

Cite this