SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

Alistair Langlands; Hamze Beati; H- Arno J Müller

doi:10.1007/978-1-0716-2863-8_15

SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

Alistair Langlands
, Hamze Beati
, H- Arno J Müller (Lead / Corresponding author)

School of Life Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

1 Citation (Scopus)

Abstract

The fruit fly Drosophila melanogaster represents a classic genetic model organism that is amenable to a plethora of comprehensive analyses including proteomics. SILAC-based quantitative proteomics is a powerful method to investigate the translational and posttranslational regulation ongoing in cells, tissues, organs, and whole organisms. Here we describe a protocol for routine SILAC labeling of Drosophila adults within one generation to produce embryos with a labeling efficiency of over 92%. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.

Original language	English
Title of host publication	SILAC
Subtitle of host publication	Methods and Protocols
Editors	Jose L. Luque-Garcia
Place of Publication	New York
Publisher	Humana Press
Pages	187-198
Number of pages	12
Edition	1
ISBN (Electronic)	9781071628638
ISBN (Print)	9781071628621
DOIs	https://doi.org/10.1007/978-1-0716-2863-8_15
Publication status	Published - 2023

Publication series

Name	Methods in Molecular Biology
Volume	2603
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Animals
Proteomics/methods
Isotope Labeling/methods
Drosophila melanogaster/genetics
Drosophila
Protein Processing, Post-Translational
Posttranslational modification
Embryo
Proteomics
Development
SILAC

ASJC Scopus subject areas

Genetics
Molecular Biology

Access to Document

10.1007/978-1-0716-2863-8_15

Fingerprints Proteomics Facility
Centre for Advanced Scientific Technologies
Facility/equipment: Facility

Cite this

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title = "SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos",

abstract = "The fruit fly Drosophila melanogaster represents a classic genetic model organism that is amenable to a plethora of comprehensive analyses including proteomics. SILAC-based quantitative proteomics is a powerful method to investigate the translational and posttranslational regulation ongoing in cells, tissues, organs, and whole organisms. Here we describe a protocol for routine SILAC labeling of Drosophila adults within one generation to produce embryos with a labeling efficiency of over 92\%. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.",

keywords = "Animals, Proteomics/methods, Isotope Labeling/methods, Drosophila melanogaster/genetics, Drosophila, Protein Processing, Post-Translational, Posttranslational modification, Embryo, Proteomics, Development, SILAC",

author = "Alistair Langlands and Hamze Beati and M{\"u}ller, \{H- Arno J\}",

note = "Copyright: {\textcopyright} 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

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doi = "10.1007/978-1-0716-2863-8\_15",

language = "English",

isbn = "9781071628621",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "187--198",

editor = "Luque-Garcia, \{Jose L.\}",

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}

SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos. / Langlands, Alistair; Beati, Hamze; Müller, H- Arno J (Lead / Corresponding author).
SILAC: Methods and Protocols. ed. / Jose L. Luque-Garcia. 1. ed. New York: Humana Press, 2023. p. 187-198 (Methods in Molecular Biology; Vol. 2603).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

AU - Langlands, Alistair

AU - Beati, Hamze

AU - Müller, H- Arno J

PY - 2023

Y1 - 2023

N2 - The fruit fly Drosophila melanogaster represents a classic genetic model organism that is amenable to a plethora of comprehensive analyses including proteomics. SILAC-based quantitative proteomics is a powerful method to investigate the translational and posttranslational regulation ongoing in cells, tissues, organs, and whole organisms. Here we describe a protocol for routine SILAC labeling of Drosophila adults within one generation to produce embryos with a labeling efficiency of over 92%. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.

AB - The fruit fly Drosophila melanogaster represents a classic genetic model organism that is amenable to a plethora of comprehensive analyses including proteomics. SILAC-based quantitative proteomics is a powerful method to investigate the translational and posttranslational regulation ongoing in cells, tissues, organs, and whole organisms. Here we describe a protocol for routine SILAC labeling of Drosophila adults within one generation to produce embryos with a labeling efficiency of over 92%. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.

KW - Animals

KW - Proteomics/methods

KW - Isotope Labeling/methods

KW - Drosophila melanogaster/genetics

KW - Drosophila

KW - Protein Processing, Post-Translational

KW - Posttranslational modification

KW - Embryo

KW - Proteomics

KW - Development

KW - SILAC

U2 - 10.1007/978-1-0716-2863-8_15

DO - 10.1007/978-1-0716-2863-8_15

M3 - Chapter (peer-reviewed)

C2 - 36370280

SN - 9781071628621

T3 - Methods in Molecular Biology

SP - 187

EP - 198

BT - SILAC

A2 - Luque-Garcia, Jose L.

PB - Humana Press

CY - New York

ER -

SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Equipment

Fingerprints Proteomics Facility

Cite this