Silver accumulation in Pseudomonas stutzeri AG259

Geoffrey M. Gadd (Lead / Corresponding author), Oliver S. Laurence, Paul A. Briscoe, Jack T. Trevors

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)

    Abstract

    Silver toxicity to Pseudomonas stutzeri AG259 was strongly dependent on the NaCl concentration in the medium, which reduced the availability of Ag+ by precipitation as AgCl. Accumulation of Ag+ by growing cultures was low being ≤ 7.5 nmol (mg dry mass)-1 over all treatments examined. The presence of NaCl in the growth medium did not markedly affect the amounts of Ag accumulated by the cells but influenced toxicity as manifest by a lag period which was greatest at low NaCl concentrations (≤ 0.1% mass/vol.). In NaCl-free medium, P. stutzeri did not grow in the presence of 0.5 mM AgNO3 in contrast to Ag-free controls. The majority of Ag accumulation by resting cells of P. stutzeri occurred within 1 min of incubation and there was little difference in uptake capacities between cells previously grown in the absence or presence of AgNO3. Lowest amounts of Ag uptake by resting cells occurred when suspended in 1 mM Mes pH 6.5, containing 1% (mass/vol.) NaCl. Prior exposure of P. stutzeri to Cu(NO3)2 resulted in a marked reduction in Ag uptake when suspended in 1 mM Mes pH 6.5, containing 0.5 mM AgNO3.

    Original languageEnglish
    Pages (from-to)168-173
    Number of pages6
    JournalBiology of Metals
    Volume2
    Issue number3
    DOIs
    Publication statusPublished - Sept 1989

    Keywords

    • Pseudomonas stutzeri AG259
    • Silver accumulation
    • Silver toxicity

    ASJC Scopus subject areas

    • General Biochemistry,Genetics and Molecular Biology
    • General Agricultural and Biological Sciences

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