SIMcheck: A toolbox for successful super-resolution structured illumination microscopy

Graeme Ball, Justin Demmerle, Rainer Kaufmann, Ilan Davis, Ian M. Dobbie, Lothar Schermelleh

Research output: Contribution to journalArticle

101 Citations (Scopus)
113 Downloads (Pure)

Abstract

Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology.

Original languageEnglish
Article number15915
Pages (from-to)1-12
Number of pages12
JournalScientific Reports
Volume5
Early online date1 Oct 2015
DOIs
Publication statusPublished - 3 Nov 2015

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    Ball, G., Demmerle, J., Kaufmann, R., Davis, I., Dobbie, I. M., & Schermelleh, L. (2015). SIMcheck: A toolbox for successful super-resolution structured illumination microscopy. Scientific Reports, 5, 1-12. [15915]. https://doi.org/10.1038/srep15915