Projects per year
Abstract
Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology.
Original language | English |
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Article number | 15915 |
Pages (from-to) | 1-12 |
Number of pages | 12 |
Journal | Scientific Reports |
Volume | 5 |
Early online date | 1 Oct 2015 |
DOIs | |
Publication status | Published - 3 Nov 2015 |
ASJC Scopus subject areas
- General
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Dive into the research topics of 'SIMcheck: A toolbox for successful super-resolution structured illumination microscopy'. Together they form a unique fingerprint.Projects
- 2 Finished
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Strategic Award: Wellcome Trust Technology Platform
Blow, J. (Investigator), Lamond, A. (Investigator) & Owen-Hughes, T. (Investigator)
1/01/13 → 30/09/18
Project: Research
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Dynamics of Fundamental Cellular Processes by Live Cell and Tissue Imaging
MacDonald, M. (Investigator), McGloin, D. (Investigator), McKenna, S. (Investigator), Storey, K. (Investigator), Swedlow, J. (Investigator) & Weijer, K. (Investigator)
1/01/13 → 31/12/17
Project: Research