SIMcheck: A toolbox for successful super-resolution structured illumination microscopy

Graeme Ball, Justin Demmerle, Rainer Kaufmann, Ilan Davis, Ian M. Dobbie, Lothar Schermelleh

Research output: Contribution to journalArticlepeer-review

144 Citations (Scopus)
104 Downloads (Pure)

Abstract

Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology.

Original languageEnglish
Article number15915
Pages (from-to)1-12
Number of pages12
JournalScientific Reports
Volume5
Early online date1 Oct 2015
DOIs
Publication statusPublished - 3 Nov 2015

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