SINHCAF/FAM60A and SIN3A specifically repress HIF 2α expression

John Biddlestone, Michael Batie, Daniel Bandarra, Ivan Munoz, Sonia Rocha (Lead / Corresponding author)

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Abstract

The SIN3A-HDAC complex is a master transcriptional repressor, required for development but often deregulated in disease. Here, we report that the recently identified new component of this complex, SINHCAF/FAM60A, links the SIN3A-HDAC co-repressor complex function to the hypoxia response. We show that SINHCAF specifically repress HIF 2α mRNA and protein expression, via its interaction with the transcription factor SP1, and recruitment of HDAC1 to the HIF 2α promoter. SINHCAF control over HIF 2α results in functional cellular changes in in vitro angiogenesis, and viability. Our analysis reveals an unexpected link between SINHCAF and the regulation of the hypoxia response.

Original languageEnglish
Pages (from-to)2073-2090
Number of pages18
JournalBiochemical Journal
Volume475
Issue number12
Early online date21 May 2018
DOIs
Publication statusPublished - 29 Jun 2018

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Biddlestone, J., Batie, M., Bandarra, D., Munoz, I., & Rocha, S. (2018). SINHCAF/FAM60A and SIN3A specifically repress HIF 2α expression. Biochemical Journal, 475(12), 2073-2090. https://doi.org/10.1042/BCJ20170945