Sinus histiocytes in axillary lymph nodes from patients with breast cancer: macrophage characteristics and activation level

R. J. C. Steele, M. Brown, O. Eremin

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Cell suspensions from 16 tumour-free axillary lymph nodes from breast cancer patients were prepared, using collagenase digestion to free the sinus histiocytes from the fibrous stroma of the nodes. The histiocytic cells so obtained were then characterized using four surface markers: Fc(IgG) receptors, C3 receptors, DR antigen and a macrophage-associated antigen (defined by the monoclonal antibody VEP-7). In addition phagocytosis was assessed using IgG-coated red cells, and both lysozyme and alpha-1-antitrypsin were localized by means of immunoperoxidase staining. The results demonstrated that the majority of sinus histiocytes carried surface macrophage markers, but that a minority displayed phagocytosis and the presence of lysozyme or alpha-1-antitrypsin.
    Original languageEnglish
    Pages (from-to)219-223
    Number of pages5
    JournalEuropean Journal of Cancer and Clinical Oncology
    Volume22
    Issue number2
    DOIs
    Publication statusPublished - 1986

    Keywords

    • Antigens, Surface
    • Breast Neoplasms
    • Female
    • HLA-DR Antigens
    • Histiocytes
    • Histocompatibility Antigens Class II
    • Humans
    • Lymph Nodes
    • Macrophage-1 Antigen
    • Macrophages
    • Muramidase
    • Phagocytosis
    • Receptors, Complement
    • Receptors, Fc
    • alpha 1-Antitrypsin

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