SKK4, a novel activator of stress-activated protein kinase-1

Sean Lawler, Ana Cuenda, Michel Goedert, Philip Cohen

    Research output: Contribution to journalArticlepeer-review

    46 Citations (Scopus)


    A cDNA was cloned and expressed that encodes human stress-activated protein kinase kinase-4 (SKK4), a novel MAP kinase kinase family member whose mRNA is widely expressed in human tissues. SKK4 activated SAPK1/JNK in vitro, but not SAPK2a/p38, SAPK2b/p38β, SAPK3/ERK6 or SAPK4. It appears to be the mammalian homologue of HEP, an activator of SAPK1/JNK in Drosophila. In human epithelial KB cells SKK4 and SKK1/MKK4 (another activator of SAPK1/ JNK) were both activated by stressful stimuli, but only SKK4 was activated by proinflammatory cytokines. The identification of SKK4 explains why the major SAPK1/JNK activator detected in many mammalian cell extracts is chromatographically separable from SKK1/MKK4.

    Original languageEnglish
    Pages (from-to)153-158
    Number of pages6
    JournalFEBS Letters
    Issue number1
    Publication statusPublished - 1 Sept 1997


    • Cytokine
    • JNK
    • MAP kinase
    • p38
    • SAPK
    • Stress

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology


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