Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation

Lynn Burchell, Viduth K. Chaugule, Helen Walden (Lead / Corresponding author)

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    24 Citations (Scopus)

    Abstract

    Parkin is an E3 ubiquitin ligase, mutations in which cause Autosomal Recessive Parkinson's Disease. Many studies aimed at understanding Parkin function, regulation and dysfunction are performed using N-terminal epitope tags. We report here that the use of small tags such as FLAG, cMyc and HA, influence the physical stability and activity of Parkin in and out of cells, perturbing the autoinhibited native state of Parkin, resulting in an active-for-autoubiquitination species.

    Original languageEnglish
    Article numbere34748
    Number of pages6
    JournalPLoS ONE
    Volume7
    Issue number4
    DOIs
    Publication statusPublished - 4 Apr 2012

    Keywords

    • MITOCHONDRIA
    • GENE
    • PROTEIN LIGASE
    • MITOPHAGY
    • PATHOGENIC MUTATIONS
    • PHOSPHORYLATION
    • PINK1
    • CELLS
    • DISEASE
    • RECESSIVE JUVENILE PARKINSONISM

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