Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation

Lynn Burchell; Viduth K. Chaugule; Helen Walden

doi:10.1371/journal.pone.0034748

Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation

Lynn Burchell, Viduth K. Chaugule, Helen Walden (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Parkin is an E3 ubiquitin ligase, mutations in which cause Autosomal Recessive Parkinson's Disease. Many studies aimed at understanding Parkin function, regulation and dysfunction are performed using N-terminal epitope tags. We report here that the use of small tags such as FLAG, cMyc and HA, influence the physical stability and activity of Parkin in and out of cells, perturbing the autoinhibited native state of Parkin, resulting in an active-for-autoubiquitination species.

Original language	English
Article number	e34748
Number of pages	6
Journal	PLoS ONE
Volume	7
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0034748
Publication status	Published - 4 Apr 2012

Keywords

MITOCHONDRIA
GENE
PROTEIN LIGASE
MITOPHAGY
PATHOGENIC MUTATIONS
PHOSPHORYLATION
PINK1
CELLS
DISEASE
RECESSIVE JUVENILE PARKINSONISM

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title = "Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation",

abstract = "Parkin is an E3 ubiquitin ligase, mutations in which cause Autosomal Recessive Parkinson's Disease. Many studies aimed at understanding Parkin function, regulation and dysfunction are performed using N-terminal epitope tags. We report here that the use of small tags such as FLAG, cMyc and HA, influence the physical stability and activity of Parkin in and out of cells, perturbing the autoinhibited native state of Parkin, resulting in an active-for-autoubiquitination species.",

keywords = "MITOCHONDRIA, GENE, PROTEIN LIGASE, MITOPHAGY, PATHOGENIC MUTATIONS, PHOSPHORYLATION, PINK1, CELLS, DISEASE, RECESSIVE JUVENILE PARKINSONISM",

author = "Lynn Burchell and Chaugule, {Viduth K.} and Helen Walden",

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AU - Burchell, Lynn

AU - Chaugule, Viduth K.

AU - Walden, Helen

PY - 2012/4/4

Y1 - 2012/4/4

N2 - Parkin is an E3 ubiquitin ligase, mutations in which cause Autosomal Recessive Parkinson's Disease. Many studies aimed at understanding Parkin function, regulation and dysfunction are performed using N-terminal epitope tags. We report here that the use of small tags such as FLAG, cMyc and HA, influence the physical stability and activity of Parkin in and out of cells, perturbing the autoinhibited native state of Parkin, resulting in an active-for-autoubiquitination species.

AB - Parkin is an E3 ubiquitin ligase, mutations in which cause Autosomal Recessive Parkinson's Disease. Many studies aimed at understanding Parkin function, regulation and dysfunction are performed using N-terminal epitope tags. We report here that the use of small tags such as FLAG, cMyc and HA, influence the physical stability and activity of Parkin in and out of cells, perturbing the autoinhibited native state of Parkin, resulting in an active-for-autoubiquitination species.

KW - MITOCHONDRIA

KW - GENE

KW - PROTEIN LIGASE

KW - MITOPHAGY

KW - PATHOGENIC MUTATIONS

KW - PHOSPHORYLATION

KW - PINK1

KW - CELLS

KW - DISEASE

KW - RECESSIVE JUVENILE PARKINSONISM

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DO - 10.1371/journal.pone.0034748

M3 - Article

SN - 1932-6203

VL - 7

JO - PLoS ONE

JF - PLoS ONE

IS - 4

M1 - e34748

ER -

Small, N-terminal tags activate Parkin E3 ubiquitin ligase activity by disrupting its autoinhibited conformation

Abstract

Keywords

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