Small RNAs derived from the 5' end of tRNA can inhibit protein translation in human cells

Andrew Sobala; Gyorgy Hutvagner

doi:10.4161/rna.24285

Small RNAs derived from the 5' end of tRNA can inhibit protein translation in human cells

Andrew Sobala, Gyorgy Hutvagner (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

268 Citations (Scopus)

Abstract

Recently, it has been shown that tRNA molecules can be processed into small RNAs that are derived from both the 5' and 3' termini. To date, the function of these tRNA fragments (tRFs) derived from the 5' end of tRNA has not been investigated in depth. We present evidence that conserved residues in tRNA, present in all 5' tRFs, can inhibit the process of protein translation without the need for complementary target sites in the mRNA. These results implicate 5' tRFs in a new mechanism of gene regulation by small RNAs in human cells.

Original language	English
Pages (from-to)	553-563
Number of pages	11
Journal	RNA Biology
Volume	10
Issue number	4
DOIs	https://doi.org/10.4161/rna.24285
Publication status	Published - Apr 2013

Keywords

Argonaute Proteins
Gene Expression Regulation
Genes, Reporter
HeLa Cells
Humans
MicroRNAs
Protein Biosynthesis
RNA, Messenger
RNA, Small Untranslated
RNA, Transfer
Transfection

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10.4161/rna.24285

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title = "Small RNAs derived from the 5' end of tRNA can inhibit protein translation in human cells",

abstract = "Recently, it has been shown that tRNA molecules can be processed into small RNAs that are derived from both the 5' and 3' termini. To date, the function of these tRNA fragments (tRFs) derived from the 5' end of tRNA has not been investigated in depth. We present evidence that conserved residues in tRNA, present in all 5' tRFs, can inhibit the process of protein translation without the need for complementary target sites in the mRNA. These results implicate 5' tRFs in a new mechanism of gene regulation by small RNAs in human cells.",

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T1 - Small RNAs derived from the 5' end of tRNA can inhibit protein translation in human cells

AU - Sobala, Andrew

AU - Hutvagner, Gyorgy

PY - 2013/4

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N2 - Recently, it has been shown that tRNA molecules can be processed into small RNAs that are derived from both the 5' and 3' termini. To date, the function of these tRNA fragments (tRFs) derived from the 5' end of tRNA has not been investigated in depth. We present evidence that conserved residues in tRNA, present in all 5' tRFs, can inhibit the process of protein translation without the need for complementary target sites in the mRNA. These results implicate 5' tRFs in a new mechanism of gene regulation by small RNAs in human cells.

AB - Recently, it has been shown that tRNA molecules can be processed into small RNAs that are derived from both the 5' and 3' termini. To date, the function of these tRNA fragments (tRFs) derived from the 5' end of tRNA has not been investigated in depth. We present evidence that conserved residues in tRNA, present in all 5' tRFs, can inhibit the process of protein translation without the need for complementary target sites in the mRNA. These results implicate 5' tRFs in a new mechanism of gene regulation by small RNAs in human cells.

KW - Argonaute Proteins

KW - Gene Expression Regulation

KW - Genes, Reporter

KW - HeLa Cells

KW - Humans

KW - MicroRNAs

KW - Protein Biosynthesis

KW - RNA, Messenger

KW - RNA, Small Untranslated

KW - RNA, Transfer

KW - Transfection

U2 - 10.4161/rna.24285

DO - 10.4161/rna.24285

M3 - Article

C2 - 23563448

SN - 1547-6286

VL - 10

SP - 553

EP - 563

JO - RNA Biology

JF - RNA Biology

IS - 4

ER -

Small RNAs derived from the 5' end of tRNA can inhibit protein translation in human cells

Abstract

Keywords

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